Articles
FACTORS AFFECTING IN VITRO TUBER FORMATION OF CALADIUM HUMBOLDTII SCHOTT. ‘PHRAYA SAVET’
Article number
755_46
Pages
351 – 356
Language
English
Abstract
Preliminary study on in vitro tuber induction of Caladium humboldtii (Raf.) Schott. Phraya Savet was successfully carried out.
An individual single shoot, with two-to-three leaves, from multiple shoot culture, was cultured for 6 weeks on modified MS (Murashige and Skoog, 1962) medium supplemented with 10-35 µM of N6-benzyladenine (BA) or 20-100 g L-1 sucrose and 5.8 g L-1 agar, in the presence or absence of 5 g L-1 activated charcoal (AC). From MS medium containing BA (MS+BA medium), AC resulted in significantly decreased tuber growth on both tuber diameter (2.84-3.31 mm/tuber) and tuber fresh weight (30.1-35.3 mg/tuber). In contrast, the absent of AC in MS+BA medium provided larger tuber diameter and heavier fresh weight with 3.40-4.68 mm/tuber and 39.1-78.0 mg/tuber, respectively.
However, the highest diameter and fresh weight of tuber were obtained from 10-20 µM BA. For MS medium added with sucrose (MS+Suc medium), there were no significant differences among 20-100 g L-1 sucrose in the absence of AC on fresh weight (27.3-37.3 mg/tuber) and diameter (2.75-3.28 mm/tuber). The great significant tuber induction medium was MS+Suc medium supplemented with 80 g
L-1 sucrose in the presence of 5 g L-1 AC, providing 198.7 mg fresh weight per tuber and 5.95 mm diameter per tuber.
An individual single shoot, with two-to-three leaves, from multiple shoot culture, was cultured for 6 weeks on modified MS (Murashige and Skoog, 1962) medium supplemented with 10-35 µM of N6-benzyladenine (BA) or 20-100 g L-1 sucrose and 5.8 g L-1 agar, in the presence or absence of 5 g L-1 activated charcoal (AC). From MS medium containing BA (MS+BA medium), AC resulted in significantly decreased tuber growth on both tuber diameter (2.84-3.31 mm/tuber) and tuber fresh weight (30.1-35.3 mg/tuber). In contrast, the absent of AC in MS+BA medium provided larger tuber diameter and heavier fresh weight with 3.40-4.68 mm/tuber and 39.1-78.0 mg/tuber, respectively.
However, the highest diameter and fresh weight of tuber were obtained from 10-20 µM BA. For MS medium added with sucrose (MS+Suc medium), there were no significant differences among 20-100 g L-1 sucrose in the absence of AC on fresh weight (27.3-37.3 mg/tuber) and diameter (2.75-3.28 mm/tuber). The great significant tuber induction medium was MS+Suc medium supplemented with 80 g
L-1 sucrose in the presence of 5 g L-1 AC, providing 198.7 mg fresh weight per tuber and 5.95 mm diameter per tuber.
Authors
R. Sugaram, C. Thapsithar, A. Thongpukdee
Keywords
Caladium humboldtii, tuber formation, N6-benzlyadenine, sucrose, activated charcoal, in vitro culture
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