Articles
DNA PROFILING OF THE GRAPE VARIETIES GROWN IN INDIA USING ISSR MARKERS
Article number
785_17
Pages
147 – 152
Language
English
Abstract
The study of genetic relationships in grapes is not only important for breeding but also for evolutionary studies and germplasm conservation.
In the present study, Inter Simple Sequence Repeat (ISSR) markers were used for the DNA profiling and characterization of total 143 grape varieties and rootstocks.
A set of selected polymorphic primers was used to generate DNA amplification profiles.
The size of amplification products ranged from 300 to 2000 bp and the number of polymorphic bands from 3–22. Cluster analysis resulted into separation of varieties belonging to vinifera and labrusca. Separate groups based on the berry colour were generally observed.
Varieties belonging to V. vinifera appeared more diverse and formed distinct sub-clusters while varieties belonging to V. labrusca showed homogeneity.
Among 43 seedless varieties, a high degree of similarity was observed.
Seeded grape varieties showed more polymorphism than seedless ones.
Among the 26 rootstocks analyzed, three main clusters were observed which were mostly according to the original species from which the rootstocks were derived.
Thus, the ISSR markers appear to be reliable and efficient for the assessment of genetic relationships among grape varieties.
In the present study, Inter Simple Sequence Repeat (ISSR) markers were used for the DNA profiling and characterization of total 143 grape varieties and rootstocks.
A set of selected polymorphic primers was used to generate DNA amplification profiles.
The size of amplification products ranged from 300 to 2000 bp and the number of polymorphic bands from 3–22. Cluster analysis resulted into separation of varieties belonging to vinifera and labrusca. Separate groups based on the berry colour were generally observed.
Varieties belonging to V. vinifera appeared more diverse and formed distinct sub-clusters while varieties belonging to V. labrusca showed homogeneity.
Among 43 seedless varieties, a high degree of similarity was observed.
Seeded grape varieties showed more polymorphism than seedless ones.
Among the 26 rootstocks analyzed, three main clusters were observed which were mostly according to the original species from which the rootstocks were derived.
Thus, the ISSR markers appear to be reliable and efficient for the assessment of genetic relationships among grape varieties.
Authors
S.A. Tamhankar, N.C. Argade, M.N. More, V.M. Dhanorkar, S.G. Patil, V.S. Rao, G.S. Karibasappa, D.C. Agrawal
Keywords
Vitis spp., polymorphism, cluster analysis, genetic relationships
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