Articles
IN VITRO BLUEBERRY (VACCINIUM SPP.) GERMPLASM MANAGEMENT IN ARGENTINA
Article number
810_78
Pages
587 – 590
Language
English
Abstract
The in vitro blueberry collection at the Concordia Experiment Station was started in 1994 when the first blueberry cultivars were introduced from the National Clonal Germplasm Repository, USDA-ARS, Corvallis, OR, USA. At present, the in vitro blueberry collection consists of 28 accessions (Southern highbush, Northern highbush and Rabbiteye) from different origins.
Under in vitro conditions it is possible to store a large number of individuals in reduced space for many months.
Storage conditions used were Woody Plant Medium without growth regulators, low temperatures (4-8°C), and low light intensity (10.8 µmol/ m2/s). The accessions were tested by ELISA for Blueberry shock virus (BlShV), Blueberry scorch virus (BlScV), Blueberry shoestring virus (BSSV), Blueberry leaf mottle virus, Peach rosette mosaic virus, Tobacco ringspot virus, and Tomato ringspot virus.
The germplasm collection has the following objectives: cultivar introduction, micropropagation, obtaining plants for field evaluation and breeding programs, and exchange material with other research centers of the country and abroad.
In vitro material has been sent to commercial and research laboratories in Argentina and Uruguay.
Also, the cultivars stored in vitro are planted in the field collection to evaluate agronomic characteristics.
Under in vitro conditions it is possible to store a large number of individuals in reduced space for many months.
Storage conditions used were Woody Plant Medium without growth regulators, low temperatures (4-8°C), and low light intensity (10.8 µmol/ m2/s). The accessions were tested by ELISA for Blueberry shock virus (BlShV), Blueberry scorch virus (BlScV), Blueberry shoestring virus (BSSV), Blueberry leaf mottle virus, Peach rosette mosaic virus, Tobacco ringspot virus, and Tomato ringspot virus.
The germplasm collection has the following objectives: cultivar introduction, micropropagation, obtaining plants for field evaluation and breeding programs, and exchange material with other research centers of the country and abroad.
In vitro material has been sent to commercial and research laboratories in Argentina and Uruguay.
Also, the cultivars stored in vitro are planted in the field collection to evaluate agronomic characteristics.
Publication
Authors
M.I. Plata, C. Anderson
Keywords
germplasm management, in vitro, blueberry, Vaccinium, Argentina
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