Articles
MANUAL PROCEDURE TO ASSESS PRO-/ANTIOXIDANT IMBALANCE IN HUMAN PLASMA BASED ON THE CROCIN BLEACHING ASSAY (CBA)
Article number
850_35
Pages
211 – 216
Language
English
Abstract
Crocin Bleaching Assay (CBA) was applied to serum samples randomly collected from 22 obese donors, in order to investigate whether it could detect patients suffering from metabolic syndrome (MS), which is associated with oxidative stress.
The same samples were also tested using the ORAC method, having analytical similarities with CBA (pH value, radical initiator and temperature) and commonly applied to biological fluids.
Statistical analysis indicated that CBA was more promising in predicting the MS in obese subjects.
The current study contributes to the commercial exploitation of saffron in the field of analytical chemistry.
The same samples were also tested using the ORAC method, having analytical similarities with CBA (pH value, radical initiator and temperature) and commonly applied to biological fluids.
Statistical analysis indicated that CBA was more promising in predicting the MS in obese subjects.
The current study contributes to the commercial exploitation of saffron in the field of analytical chemistry.
Publication
Authors
S.A. Ordoudi, N. Nenadis, M.Z. Tsimidou, M. Sarigianni, K. Paletas
Keywords
Crocin Bleaching Assay, ORAC, oxidative stress, serum
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