Articles
DETECTION OF ERYTHROSINE IN SAFFRON
Article number
850_37
Pages
225 – 230
Language
English
Abstract
Erythrosine (E127, CI 45430) is not easily detected in adulterated saffron under the TLC and HPLC conditions proposed by ISO (3632-2, 2003) specifications for identification of artificial colorants in this precious plant material.
In this study fluorescence is introduced for the first time as a means of detection after sample clean-up prior chromatographic separation according to aforementioned specifications at this stage of analysis.
The mimimum required performance limit (MRPL) was found lower than that obtained using UV-Vis spectrophotometry by two-orders of magnitude.
After chromatographic separation of erythrosine fluorescence detection at the same addition levels was less successful.
In this study fluorescence is introduced for the first time as a means of detection after sample clean-up prior chromatographic separation according to aforementioned specifications at this stage of analysis.
The mimimum required performance limit (MRPL) was found lower than that obtained using UV-Vis spectrophotometry by two-orders of magnitude.
After chromatographic separation of erythrosine fluorescence detection at the same addition levels was less successful.
Publication
Authors
S.A. Ordoudi, K. Tsioga, M.Z. Tsimidou
Keywords
Crocus sativus L., adulteration, synthetic colorants, fluorescence, photodegradation
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