Articles
ANTIOXIDANT SAFFRON CONSTITUENTS BIND DNA AND TRNA
Article number
850_36
Pages
217 – 222
Language
English
Abstract
Crocetin (CRT) and dimethylcrocetin (DMCRT) are derived from saffrons carotenoids while safranal is the main component of its essential oil.
The antioxidant activity of these compounds was tested by the DPPH antioxidant activity assay.
Also the interactions of CRT, DMCRT and safranal with calf-thymus DNA and transfer RNA in aqueous solution at physiological conditions are reported.
The structural analysis showed external binding to the major or minor groove and some degree of intercalation of saffron compounds to DNA and RNA. A partial B- to A-DNA transition occurs at high ligand concentration while tRNA remains in the A-family.
The antioxidant activity of these compounds was tested by the DPPH antioxidant activity assay.
Also the interactions of CRT, DMCRT and safranal with calf-thymus DNA and transfer RNA in aqueous solution at physiological conditions are reported.
The structural analysis showed external binding to the major or minor groove and some degree of intercalation of saffron compounds to DNA and RNA. A partial B- to A-DNA transition occurs at high ligand concentration while tRNA remains in the A-family.
Publication
Authors
C.D. Kanakis, P.A. Tarantilis, M.G. Polissiou, H.A. Tajmir-Riahi
Keywords
binding constant, binding sites, conformation, Crocus sativus L., FT-IR, UV-Visible
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