Articles
THE SELF-INCOMPATIBLITY S-ALLELES OF BRAZILIAN APPLE CULTIVARS
Article number
872_24
Pages
187 – 192
Language
English
Abstract
The purpose of this study was to identify and characterize the S-alleles of gametophytic incompatibility among apple cultivars developed in Brazil, to give support to correctly choose the combinations of parents in the apple breeding programs.
It also seeks to identify correct combinations of scion/pollinator cultivars of commercial apples.
For the molecular characterization, 16 specific S-alleles primers were tested.
The molecular analysis confronted to the reference cultivars, showed that the cultivars Daiane, Imperatriz and Princesa have the same incompatibility alleles S3 and S5, while Lisgala showed the alleles S2 and S5; Suprema, S1 and S9; Catarina, S1 and S19; Joaquina and Fred Hough, S5 and S19; Baronesa, S3 and S9; Duquesa, S2 and S3; and for Primícia and Condessa it was only possible to identify one of the S-alleles, namely S24 and S2, respectively, the second allele remaining to be identified.
It also seeks to identify correct combinations of scion/pollinator cultivars of commercial apples.
For the molecular characterization, 16 specific S-alleles primers were tested.
The molecular analysis confronted to the reference cultivars, showed that the cultivars Daiane, Imperatriz and Princesa have the same incompatibility alleles S3 and S5, while Lisgala showed the alleles S2 and S5; Suprema, S1 and S9; Catarina, S1 and S19; Joaquina and Fred Hough, S5 and S19; Baronesa, S3 and S9; Duquesa, S2 and S3; and for Primícia and Condessa it was only possible to identify one of the S-alleles, namely S24 and S2, respectively, the second allele remaining to be identified.
Authors
C.L. Albuquerque Junior, R.O. Nodari, A.C.M. Dantas, F. Denardi
Keywords
Malus × domestica, apple breeding, polymerase chain reaction
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