Articles
METABOLIC ACTIVITY EVALUATION OF TEMPERATE TREE FRUIT BUDS BY USING THE TETRAZOLIUM TEST
Article number
872_9
Pages
89 – 96
Language
English
Abstract
A way to estimate bud dormancy intensity, and to predict if breakage of dormancy in moderate winter areas is necessary, is the determination of its metabolic activity.
The objective of this work was to develop an easy, precise and quick method to evaluate metabolic activity of apple, pear, peach, plum, persimmon, grape and kiwi buds.
The method was based on the tetrazolium salt (2, 3, 5-triphenyl-tetrazolium chloride) mechanism, which functions as receptor of hydrogen ions released from cellular respiration and produces a stable, only soluble in ethylic alcohol, red substance.
Redder alcoholic solution means higher bud metabolic activity, which may be measured by absorbance with a spectrophotometer at 560 nm.
Eight to twelve 1-year-old buds were collected and longitudinally sectioned for internal tissue exposition to five concentrations (2, 4, 6, 8 and 10 g/L) of 5 ml tetrazolium solutions during five different exposition times (2, 4, 6, 8 and 10h). A completely randomized design, using 5 × 5 factorial treatments and 4 replications was used.
Results were analyzed by polynomial regression and optimal tetrazolium concentration and time exposition were detected.
Two hours exposition was suitable for all the species studied herein.
Ideal tetrazolium concentration for apple, pear, peach, plum and persimmon buds was 10 g/L and for grape and kiwi buds was 8 g/L. The proposed test is viable, quick and easy and may be used as a tool for bud dormancy evaluation by means of metabolic activity determination.
The objective of this work was to develop an easy, precise and quick method to evaluate metabolic activity of apple, pear, peach, plum, persimmon, grape and kiwi buds.
The method was based on the tetrazolium salt (2, 3, 5-triphenyl-tetrazolium chloride) mechanism, which functions as receptor of hydrogen ions released from cellular respiration and produces a stable, only soluble in ethylic alcohol, red substance.
Redder alcoholic solution means higher bud metabolic activity, which may be measured by absorbance with a spectrophotometer at 560 nm.
Eight to twelve 1-year-old buds were collected and longitudinally sectioned for internal tissue exposition to five concentrations (2, 4, 6, 8 and 10 g/L) of 5 ml tetrazolium solutions during five different exposition times (2, 4, 6, 8 and 10h). A completely randomized design, using 5 × 5 factorial treatments and 4 replications was used.
Results were analyzed by polynomial regression and optimal tetrazolium concentration and time exposition were detected.
Two hours exposition was suitable for all the species studied herein.
Ideal tetrazolium concentration for apple, pear, peach, plum and persimmon buds was 10 g/L and for grape and kiwi buds was 8 g/L. The proposed test is viable, quick and easy and may be used as a tool for bud dormancy evaluation by means of metabolic activity determination.
Authors
R.I.N. Carvalho, J.C. Rendoke, L.A. Biasi, F. Zanette
Keywords
Malus ×domestica, Pyrus communis, Prunus persica, Prunus salicina, Diospyros kaki, Vitis labrusca, Actinidia deliciosa
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