Articles
OPTIMIZATION OF THE ISSR-PCR SYSTEM FOR THE BAMBOOS OF ECONOMIC IMPORTANCE
Article number
875_71
Pages
543 – 548
Language
English
Abstract
The use of inter-simple sequence repeats (ISSR) has been reported as being more useful for assessing genetic diversity for plant populations and inferring genetic relationships among closely related cultivars compared to standard DNA sequencing methods.
In the present study, an optimal ISSR-PCR system was established for selected woody bamboos of economic importance.
This was done by testing several PCR parameters, including annealing temperature, MgCl2 concentration (1.5-2.5 mM), and primer concentration (1.5-2.5 μM). Three anchored primers were used.
Gel electrophoresis was run using 1.8 and 2.0% agarose gel.
Reproducibility of this method was tested by repeating the procedures under optimal conditions.
In the present study, an optimal ISSR-PCR system was established for selected woody bamboos of economic importance.
This was done by testing several PCR parameters, including annealing temperature, MgCl2 concentration (1.5-2.5 mM), and primer concentration (1.5-2.5 μM). Three anchored primers were used.
Gel electrophoresis was run using 1.8 and 2.0% agarose gel.
Reproducibility of this method was tested by repeating the procedures under optimal conditions.
Authors
W.L. Goh, S. Chandran, K.M. Wong
Keywords
inter-simple sequence repeats (ISSR), woody bamboos
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