Articles
OBTAINING GENOMIC DNA FOR CONSTRUCTION OF GENOMIC LIBRARY
Article number
875_72
Pages
549 – 554
Language
English
Abstract
The isolation of pure genomic DNA from the white and red dragon fruits, Hylocereus undatus and Hylocereus polyrhizus (Cactaceae) for the construction of a genomic library is described.
These vine cacti originate from northern South America, Central America and Mexico, and are cultivated as new exotic fruit crops in SE Asia.
Successful establishment of a genomic library serves as a platform for molecular studies, such as gene characterization and gene analysis, but intact and high molecular weight DNA of good quality can be difficult to obtain.
Furthermore, presence of polysaccharides in DNA extracts interferes with downstream processes such as restriction digest and polymerase chain reaction (PCR). Genomic DNA can be extracted using various plant parts from 2–3-week-old seedling leaves, roots and stems.
DNA quality is checked using a spectrophotometer and digested using BamH1. Genomic DNA so isolated had the highest quality following restriction digestion analysis.
These vine cacti originate from northern South America, Central America and Mexico, and are cultivated as new exotic fruit crops in SE Asia.
Successful establishment of a genomic library serves as a platform for molecular studies, such as gene characterization and gene analysis, but intact and high molecular weight DNA of good quality can be difficult to obtain.
Furthermore, presence of polysaccharides in DNA extracts interferes with downstream processes such as restriction digest and polymerase chain reaction (PCR). Genomic DNA can be extracted using various plant parts from 2–3-week-old seedling leaves, roots and stems.
DNA quality is checked using a spectrophotometer and digested using BamH1. Genomic DNA so isolated had the highest quality following restriction digestion analysis.
Authors
L.K.P. Daniel, Z.X. Loo, S. Chandran
Keywords
genomic library, dragon fruit, restriction digest, high molecular weight DNA, Cactaceae
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