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Articles

QUICK CHARACTERIZATION OF CITRUS TRISTEZA VIRUS ISOLATES BY CAPILLARY ELECTROPHORESIS-SINGLE-STRAND CONFORMATION POLYMORPHISM

Article number
892_22
Pages
183 – 188
Language
English
Abstract
Three SY isolates of Citrus tristeza closterovirus (CTV) found in Sicily (SG29, TDV, Tapi) were analysed by Capillary Electrophoresis-Single-Strand Conformation Polymorphism (CE-SSCP) of p18, p23 and p27 genes.
The profiles were found to belong to two groups with differences between SG29 and Tapi and TDV. This newly emerging approach has proved to be robust and highly reproducible.
The standard deviation means of the migration data for a total of 72 analyses of genes and isolates was very low (±0.046 and ±0.052 for forward and reverse strands, respectively). BLAST homology analysis of p18 and p23 genes confirmed the CE-SSCP groupings from peak positions relative to internal size markers.
According to the results, the method appears to be a powerful tool for quickly characterizing the genetic diversity of CTV isolates.
It is simple to use, highly sensitive and provides reproducible results which means a large number of samples can be processed at once.

Publication
II International Symposium on Citrus Biotechnology
Authors
D. Raspagliesi, G. Licciardello, G. Lombardo, A. Catara, S. Rizza
Keywords
CTV, CE-SSCP, genetic diversity, molecular characterization
Full text
https://doi.org/10.17660/ActaHortic.2011.892.22
Online Articles (48)
M. Talon | M. Cercos | D.J. Iglesias | J.M. Colmenero-Flores | V. Ibáñez | J. Brumos | M.A. Herrero-Ortega | G. Rios | J. Terol | F.R. Tadeo
T. Shimizu | H. Fujii | N. Kotoda | K. Yano | T. Endo
A. Aprile | C. Federici | T. Close | M. Roose | L. De Bellis | L. Cattivelli
Chunxian Chen | F.G. Gmitter Jr.
A. Uzun | T. Yesiloglu | Y. Aka Kacar | O. Tuzcu | O. Gulsen | G. Kafa | B. Yildirim | S. Anıl
A. Asadi Abkenar | M. Mardi | R. Matsumoto
A. Uzun | T. Yesiloglu | Y. Aka Kacar | O. Tuzcu | O. Gulsen | U. Seday | I. Canan
M.A. Germanà | S. Messina | G. Di Cara | N.N. Albanese | A. Pivetti | I. Pucci-Minafra
E.M. Koutsioumari | A.E. Voloudakis
O. Simsek | Y. Aka Kacar | T. Yesiloglu | P. Ollitrault
B. Tan | W.W. Guo
J.W. Grosser | M. Dutt | A. Omar | V. Orbovic | G. Barthe
E.U. Khan | X.Z. Fu | J.H. Liu
M. Dutt | J.W. Grosser
P. Caruso | D. Pietro Paolo | M.R. Paolì | G. Reforgiato Recupero | M. Mattana | A. Genga | I. Coraggio | F. Locatelli
S. La Malfa | G. Distefano | F. Domina | E. Nicolosi | V. Toscano | A. Gentile
A.R. Lo Piero | I. Puglisi | V. Mercurio | G. Petrone
J.X. Shi | E.E. Goldschmidt | R. Goren | R. Porat
E. Pagán | A. Pérez-Pastor | R. Pérez-Reverte | A.B. Provencio | J.A. Martínez-López | R. Domingo | J.M. Robles | J.P. Fernández-Trujillo
L. Qin | S.L. Luo | Z.N. Deng | J.W. Yan | N. Li | F.R. Yuan
G. McCollum | R. Stange | U. Albrecht | K. Bowman | R. Niedz | E. Stover
D. Raspagliesi | G. Licciardello | G. Lombardo | A. Catara | S. Rizza
B.N. Chakraborty | U. Chakraborty | K. Rai | S. Allay | P.L. Dey
M. Russo | F.M. Grasso | P. Bella | G. Licciardello | A. Catara | V. Catara
U. Chakraborty | B.N. Chakraborty | S. Allay | U. De | A.P. Chakraborty
M. Tessitori | M. Davino | J.P. Thermoz | S. Davino
G. Polizzi | D. Aiello | I. Castello | A. Vitale | J.Z. Groenewald | P.W. Crous
P. Bella | G. Ialacci | M. Russo | A. Catara | V. Catara
R.K. Yokomi | M. Saponari | G. Vidalakis
A. Lombardo | G. Licciardello | A. Bertuccio | G. Vistoli | A. Pedretti
V. Cavalieri | G. Mazzeo | G. Tropea Garzia | A. Russo | E. Buonocore
G. Scuderi | S. Panebianco | C. Platania | G. Cirvilleri
T. Kapari-Isaia | A.E. Voloudakis | A. Kyriakou | I. Ioannides | L. Papayiannis | S. Samouel | E.M. Koutsioumari | A. Georgiou | G. Minas
D. Padoan | B. Chiancone | M.A. Germanà
C.I. Tallón | I. Porras | O. Pérez-Tornero
S. Recupero | G. Russo | G. Reforgiato Recupero | D. Pietro Paolo | F. Caldarera
Y. Aka Kacar | Y. Yalcin Mendi | O. Simsek | T. Yesiloglu | M. Boncuk
M.A. Germanà | M. Lambardi | E.A. Ozudogru
L. Zacarias | B. Alquezar | M.J. Rodrigo
G. Distefano | M. Caruso | G. Las Casas | S. La Malfa | A. Gentile | E. Tribulato | O. Simsek | P. Rapisarda
T. Azoulay-Shemer | S. Harpaz-Saad | Y. Eyal | E.E. Goldschmidt
C. Licciardello | M.P. Russo | G. Reforgiato Recupero | V. Muccilli | V. Cunsolo | R. Saletti | S. Foti | D. Fontanini
P. Romero | M.J. Rodrigo | F. Alférez | L. Zacarias | M.T. Lafuente
C. Licciardello | M.P. Russo | G. Reforgiato Recupero | J. Bernardi | A. Marocco | E. Butelli | C. Martin | M.L. Chiusano
Chunxian Chen | F.G. Gmitter Jr.
Z. Tietel | E. Fallik | R. Porat | E. Lewinsohn | E. Bar
S. Canterino | D. Donno | G.L. Beccaro | G. Bounous
F. Licciardello | G. Muratore | C. Avola | F. Tomaselli | E. Maccarone