Articles
IN VITRO PROPAGATION OF HYBRID CANDIDATE ROOTSTOCKS FOR ORNAMENTAL CITRUS
Article number
892_36
Pages
301 – 304
Language
English
Abstract
Some hybrids obtained in 2005 and 2006 crossing chinotto small leaf (Citrus myrtifolia Raf.) with trifoliate orange [Poncirus trifoliate (L.) Raf.], Flying Dragon trifoliate orange [P. trifoliate var. monstrosa (T. Ito) Swing.] and Troyer citrange (P. trifoliata × C. sinensis) were developped with the aim to test them as candidate rootstocks able to limit the canopy growth for ornamental use.
The long juvenile period (at least 5 years to start blooming) makes conventional breeding in citrus unusually slow.
The aim of this work is to establish a procedure in vitro culture to shorten this time by propagation system.
Some budsticks of six greenhouse-grown hybrids were used as the source of 20-30 nodal segments 1.0-1.5 cm in length and cultured in MS containing 3 mg/L
6-benzylaminopurine.
Three weeks later the shoots were transferred to a medium multiplication containing 0.5 mg/L of 6-benzylaminopurine and 0.25 mg/L of indole-3-butyric acid and, 60 days after, in an elongation medium including 1.0 mg/L gibberellic acid.
Root formation was subsequently obtained in a mix of peat and expanded perlite.
At present several saplings of each rootstock are cultivated under greenhouse to be grafted within one year.
The long juvenile period (at least 5 years to start blooming) makes conventional breeding in citrus unusually slow.
The aim of this work is to establish a procedure in vitro culture to shorten this time by propagation system.
Some budsticks of six greenhouse-grown hybrids were used as the source of 20-30 nodal segments 1.0-1.5 cm in length and cultured in MS containing 3 mg/L
6-benzylaminopurine.
Three weeks later the shoots were transferred to a medium multiplication containing 0.5 mg/L of 6-benzylaminopurine and 0.25 mg/L of indole-3-butyric acid and, 60 days after, in an elongation medium including 1.0 mg/L gibberellic acid.
Root formation was subsequently obtained in a mix of peat and expanded perlite.
At present several saplings of each rootstock are cultivated under greenhouse to be grafted within one year.
Authors
S. Recupero, G. Russo, G. Reforgiato Recupero, D. Pietro Paolo, F. Caldarera
Keywords
breeding, regeneration, multiplication
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