Articles
CHARACTERIZATION OF ALTERNARIA ALTERNATE ISOLATES FROM TANGERINE HYBRIDS AFFECTED BY BROWN SPOT IN ITALY
Article number
892_28
Pages
245 – 250
Language
English
Abstract
Alternaria brown spot, caused by Alternaria alternate, is the most important disease of tangerines and their hybrids worldwide, including Italy.
The disease represents, for some susceptible citrus cultivar, a limiting factor in profitable cultivation.
The results of the characterization of A. alternate isolates obtained from lesions on leaves and fruits of Fortune, Nova and Winola hybrids and Valencia sweet orange, collected in two groves located in Calabria and Sicily, is reported.
An endopolygalacturonase gene (endoPG) was sequenced from five representative strains and a phylogenetic tree based on endoPG sequence data of Italian isolates and those of citrus associated Alternaria species deposited in Genbank was constructed.
Italian brown spot isolates were grouped both in clusters with brown spot isolates from different countries and in clusters with A. alternate isolates associated with other diseases of citrus.
Intra-population variability was demonstrated by a fAFLP protocol.
A primer pair combination was selected amongst four primer combinations and tested on 18 A. alternate isolates.
Mixed populations of A. alternate were recovered in a single citrus grove in Calabria.
No correlation was found among geographical origin, host, and year of isolation.
The disease represents, for some susceptible citrus cultivar, a limiting factor in profitable cultivation.
The results of the characterization of A. alternate isolates obtained from lesions on leaves and fruits of Fortune, Nova and Winola hybrids and Valencia sweet orange, collected in two groves located in Calabria and Sicily, is reported.
An endopolygalacturonase gene (endoPG) was sequenced from five representative strains and a phylogenetic tree based on endoPG sequence data of Italian isolates and those of citrus associated Alternaria species deposited in Genbank was constructed.
Italian brown spot isolates were grouped both in clusters with brown spot isolates from different countries and in clusters with A. alternate isolates associated with other diseases of citrus.
Intra-population variability was demonstrated by a fAFLP protocol.
A primer pair combination was selected amongst four primer combinations and tested on 18 A. alternate isolates.
Mixed populations of A. alternate were recovered in a single citrus grove in Calabria.
No correlation was found among geographical origin, host, and year of isolation.
Authors
P. Bella, G. Ialacci, M. Russo, A. Catara, V. Catara
Keywords
Alternaria brown spot, small-spored Alternaria, tangerine pathotype, endoPG gene, fAFLP
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