Articles
EXPRESSION OF THE CHRYSANTHEMUM VIRUS B COAT PROTEIN GENE IN TRANSGENIC CHRYSANTHEMUM
Article number
901_11
Pages
95 – 98
Language
English
Abstract
The most promising strategy to create virus resistant plants today is gene silencing technology called RNA interference.
The essence of RNA-induced gene silencing is the delivery of double-stranded RNA into an organism or cell, inducing a sequence-specific RNA degradation mechanism that effectively silences a targeted gene.
For this purpose we transferred the Chrysanthemum virus B coat protein gene sequences into specific vector pKannibal in order to generate small double stranded RNA complementary to this sequence and obtained four transgenic lines of chrysanthemum White Snowdon.
The essence of RNA-induced gene silencing is the delivery of double-stranded RNA into an organism or cell, inducing a sequence-specific RNA degradation mechanism that effectively silences a targeted gene.
For this purpose we transferred the Chrysanthemum virus B coat protein gene sequences into specific vector pKannibal in order to generate small double stranded RNA complementary to this sequence and obtained four transgenic lines of chrysanthemum White Snowdon.
Authors
T. Mitiouchkina, T. Skachkova, O. Shulga, S. Dolgov
Keywords
Chrysanthemum morifolium Ramat., virus resistance, coat protein, genetic transformation, Chrysanthemum virus B, antisense RNA
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