Articles
VIRUSES OF ORNAMENTAL PLANTS IN INDIA – CURRENT RESEARCH STATUS AND FUTURE PROSPECTS
Article number
901_7
Pages
67 – 76
Language
English
Abstract
India is gracefully paving its path to emerge as a significant player of the world floriculture trade and a new floral superpower of the future.
Floriculture is an industry which has tremendous potential in India.
The different types of climatic conditions provide for the possibility of growing almost all the major cut flower species of the world, either from tropical, subtropical or temperate climates.
Viruses, viroids and phytoplasma are persistent threats to the propagation of ornamental crops.
Vegetative propagation, changed cultural practices, international trade practices, and movement of planting material to newer areas with different climate leads to spread of pathogens and disease outbreaks.
To combat viral diseases it is important to diagnose the viruses efficiently and effectively.
Advances in molecular techniques have evolved immensely leading to reliable detection of these pathogens.
At IHBT about 25 viruses have been detected from floricultural crops by electron microscopy, immunosorbent electron microscopy, ELISA, PCR/RT-PCR/IC-RT-PCR and nucleic acid hybridization.
Polyclonal rabbit antibodies against several of these viruses have been developed using coat protein genes expressed in E. coli. RT-PCR has been standardized for detection of ArMV and SLRSV in their nematode vectors.
Complete genomes of CMV, CarMV, CERV, CVB, CymMV, LSV and Chrysanthemum stunt viroid (CSVd) have been characterized.
In vitro techniques have been standardized to produce virus-tested ornamental crops.
The planting material are generated and provided to the growers.
Farmers and NGO are being trained for production and maintenance of virus-free quality planting material.
IHBT is an accredited lab under the National Certification System of the Indian Department of Biotechnology for tissue culture raised plants.
Recently focus is being given to the study of host pathogen interaction, tran-scriptomics, development of cDNA chips, biosensors and understanding the role of viral suppressor molecules in disease management.
This paper describes the current research status and future prospects of viral disease management in floricultural crops.
Floriculture is an industry which has tremendous potential in India.
The different types of climatic conditions provide for the possibility of growing almost all the major cut flower species of the world, either from tropical, subtropical or temperate climates.
Viruses, viroids and phytoplasma are persistent threats to the propagation of ornamental crops.
Vegetative propagation, changed cultural practices, international trade practices, and movement of planting material to newer areas with different climate leads to spread of pathogens and disease outbreaks.
To combat viral diseases it is important to diagnose the viruses efficiently and effectively.
Advances in molecular techniques have evolved immensely leading to reliable detection of these pathogens.
At IHBT about 25 viruses have been detected from floricultural crops by electron microscopy, immunosorbent electron microscopy, ELISA, PCR/RT-PCR/IC-RT-PCR and nucleic acid hybridization.
Polyclonal rabbit antibodies against several of these viruses have been developed using coat protein genes expressed in E. coli. RT-PCR has been standardized for detection of ArMV and SLRSV in their nematode vectors.
Complete genomes of CMV, CarMV, CERV, CVB, CymMV, LSV and Chrysanthemum stunt viroid (CSVd) have been characterized.
In vitro techniques have been standardized to produce virus-tested ornamental crops.
The planting material are generated and provided to the growers.
Farmers and NGO are being trained for production and maintenance of virus-free quality planting material.
IHBT is an accredited lab under the National Certification System of the Indian Department of Biotechnology for tissue culture raised plants.
Recently focus is being given to the study of host pathogen interaction, tran-scriptomics, development of cDNA chips, biosensors and understanding the role of viral suppressor molecules in disease management.
This paper describes the current research status and future prospects of viral disease management in floricultural crops.
Authors
A.A. Zaidi, V. Hallan, G. Raikhy, A.K. Singh, R. Ram
Keywords
virus, Alstroemeria, Begonia, carnation, Chrysanthemum, Freesia, Gladiolus, Gerbera, Iris, Lilium, Lisianthus, Narcissus, orchid, Ornithogalum, Pelargonium, rose, spider lily, tuberose, Tulipa, ELISA, PCR, vector
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