Home > XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Berries: From Genomics to Sustainable Production, Quality and Health > HIGH RESOLUTION MELTING DETECTS SEQUENCE POLYMORPHISM IN RUBUS OCCIDENTALIS MONOMORPHIC MICROSATELLITE MARKERS

Articles

HIGH RESOLUTION MELTING DETECTS SEQUENCE POLYMORPHISM IN RUBUS OCCIDENTALIS MONOMORPHIC MICROSATELLITE MARKERS

Article number

926_11

Pages

91 – 95

Language

English

Abstract

Microsatellite, or simple sequence repeat (SSR), markers are valuable as co-dominant genetic markers with a variety of applications such as DNA fingerprinting, linkage mapping, and population structure analysis.
However, primer pairs designed from the regions that flank SSRs often generate fragments with no size polymorphism.
High resolution melting (HRM) technology provides a new method of identifying sequence variations in these amplified fragments.
In this study, we used HRM to identify polymorphism in SSR PCR products that were monomorphic for size in a black raspberry (Rubus occidentalis subgenus Idaeobatus) population.
When HRM revealed polymorphism between the parents of a mapping population, the seedlings were genotyped and scored.
The HRM markers segregated in a Mendelian fashion.
Comparison of DNA sequences of the parents revealed the presence of single nucleotide polymorphisms (SNP) in the amplicons.
HRM can be a valuable tool for detecting polymorphism in PCR amplicons using existing primer pairs that would otherwise be of little use.

Publication

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Berries: From Genomics to Sustainable Production, Quality and Health

Authors

M. Dossett, N.V. Bassil, C.E. Finn

Keywords

SSR, single nucleotide polymorphism, HRM, black raspberry

Full text

https://doi.org/10.17660/ActaHortic.2012.926.11

Online Articles (107)

1. A MULTINATIONAL APPROACH FOR CONSERVING THE EUROPEAN GENETIC RESOURCES OF CURRANTS AND GOOSEBERRY

2. EUROPEAN SMALL BERRIES GENETIC RESOURCES, GENBERRY: TESTING A PROTOCOL FOR DETECTING FRUIT NUTRITIONAL QUALITY IN EU STRAWBERRY GERMPLASM COLLECTIONS

3. GOOSEBERRY (RIBES GROSSULARIA L.) GENETIC RESOURCES IN LATVIA

K. Kampuss | S. Strautina | S. Kampuse | I. Krasnova

4. RECENT PROGRESS IN BERRIES BREEDING IN ROMANIA

P. Mladin | G. Mladin | M. Coman | E. Chiţu | V. Chiţu

5. RESULTS OF THE COMMON INTERNATIONAL BREEDING PROGRAM FOR BLACKCURRANT

6. SCREENING FOR RESISTANCE TO PHYTOPHTHORA ROOT ROT IN RASPBERRIES: IDENTIFYING NEW SOURCES OF RESISTANCE

C. Kempler | A.M. Muehlchen | T.A. Forge

7. STUDY OF VOLATILE COMPOUND CONTENTS IN A PROGENY ISSUED FROM CROSS BETWEEN ‘CAMAROSA’ AND ‘BENIHOPPE’

8. COMPARISON OF AROMA COMPOUNDS OF 3 NEWLY-RELEASED STRAWBERRY CULTIVARS AND THEIR PARENTS

9. VARIATION OF FRUIT TRAITS IN HIGHBUSH BLUEBERRY SEEDLINGS FROM A FACTORIAL CROSS

J. Scalzo | V. Sguigna | B. Mezzetti | J. Stanley | P. Alspach

10. ISOLATION OF DNA-METHYLTRANSFERASE GENES AND THEIR PROMOTER SEQUENCES FROM STRAWBERRY (FRAGARIA × ANANASSA DUCH.)

11. HIGH RESOLUTION MELTING DETECTS SEQUENCE POLYMORPHISM IN RUBUS OCCIDENTALIS MONOMORPHIC MICROSATELLITE MARKERS

M. Dossett | N.V. Bassil | C.E. Finn

12. FINGERPRINTING IN RASPBERRY BY SSRS ANALYSIS AND CULTIVAR CHARACTERISATION

S. Venturi | E. Galassi | L. Dondini | S. Tartarini | G. Costa

13. QUALITY, NUTRITIONAL QUALITY AND NUTRACEUTICAL VALUE AS A NEW TASK FOR STRAWBERRY BREEDING

J. Diamanti | F. Capocasa | B. Mezzetti | M. Battino | F. Giampieri

14. ISOLATION AND ANALYSIS OF 5′ REGULATORY SEQUENCE OF VVICOR11 FROM COWBERRY

Q.J. Wang | Z.H. Gao | J.M. Tao | S.C. Qu | Z. Zhang

15. EFFECT OF STRAWBERRY AND BARLEY DEFENCE PROTEINS ON NEMATODE PESTS

M. Gambardella | R. Ríos | E. Aballay | I. Cambra | I. Diaz

16. BLACK CURRANT PRODUCTION, BREEDING AND PROCESSING IN CHINA

Qin Dong | Junwei Huo | Sui Wei | Zhuangfei Zhang

17. EUROPEAN NETWORK FOR BLACKCURRANT (RIBES NIGRUM L.) CULTIVAR EVALUATION

18. EUROPEAN NETWORK FOR STRAWBERRY CULTIVAR EVALUATION

19. ‘FUENTEPINA’, A NEW STRAWBERRY CULTIVAR FROM THE SPANISH PUBLIC BREEDING PROGRAM

20. YIELD COMPONENTS AND FRUIT QUALITY OF PROMISING YELLOW FRUIT RASPBERRY SEEDLINGS

M. Fotirić Aksić | M. Nikolić | A. Radović | J. Milivojević | D. Nikolić

21. ECO-PHYSIOLOGICAL AND HORTICULTURAL CHARACTERISTICS OF TWO KOREAN WILD VACCINIUMS

Hye-Sung Cho | Youn-Sup Cho | Jeong-An Cho

22. ESTIMATION OF YIELDING AND FRUIT QUALITY OF NINE STRAWBERRY CULTIVARS

M. Bieniasz | M. Małodobry | E. Dziedzic

23. YIELD AND QUALITY CHARACTERISTICS OF SOME STRAWBERRY CULTIVARS IN ADANA, TURKEY

S. Paydaş Kargı | E. Kafkas | G. Baktemur

24. EVALUATION OF OVERALL PERFORMANCE OF ELEVEN BLACKBERRY CULTIVARS IN NANJING OF CHINA

25. PERFORMANCE OF SOME ITALIAN STRAWBERRY GENOTYPES GROWN UNDER CONDITIONS OF R. MACEDONIA

M. Kiprijanovski | T. Arsov | V. Gjamovski

26. PRODUCTIVITY OF SOME BLUEBERRY CULTIVARS UNDER LATVIA CONDITIONS

D. Sterne | M. Liepniece | M. Abolins

27. BIOLOGICAL AND POMOLOGICAL PECULIARITIES OF SOME RASPBERRY CULTIVARS

H. Hovhannisyan

28. FLOWERING AND FRUITING ON COMMAND IN BERRY CROPS

B.C. Strik

29. ARE THERE COMMON SYSTEMS FOR FLOWER BUD INITIATION IN THE GENUS RUBUS?

A. Dale | P.B. Oliveira | T. Valdivieso

30. CONTRASTING ENVIRONMENTAL FLOWERING RESPONSES IN ANNUAL AND BIENNIAL RASPBERRIES – A MINI-REVIEW

A. Sønsteby | O.M. Heide

31. RELATIONSHIP BETWEEN PLANT ARCHITECTURE AND FRUIT PRODUCTION OF THE SHORT-DAY STRAWBERRY CULTIVAR ‘GARIGUETTE’

J.P. Bosc | D. Neri | F. Massetani | A. Bardet

32. NEW METHODS FOR ADVANCING OR DELAYING ANTHESIS IN SHORT-DAY STRAWBERRY

F. Takeda

33. FLOWER DIFFERENTIATION AND PLANT ARCHITECTURE OF RASPBERRY, BLACKBERRY AND WHITE- AND REDCURRANT

D. Neri | F. Massetani | P. Zucchi | M. Giacomelli | G. Savini

34. BREAKING DORMANCY BY CYCLIC LIGHTING IN STRAWBERRY GLASSHOUSE CULTIVATION: SUSTAINABLE ALTERNATIVES FOR INCANDESCENT LAMPS

T. Van Delm | P. Melis | K. Stoffels | W. Baets

35. CHARACTERISTICS OF LINGONBERRY PLANTS PROPAGATED BY IN VITRO AND EX VITRO PROPAGATION METHODS

S.C. Debnath | P. Vyas | A.U. Igamberdiev

36. MICROPROPAGATION IN VITRO OF HIGHBUSH BLUEBERRY (VACCINIUM CORYMBOSUM L.)

D. Ruzić | T. Vujović | R. Cerović | M.G. Ostrolucka | A. Gajdosova

37. ROOTING OF BLUEBERRY HARDWOOD CUTTINGS AS AFFECTED BY WOOD TYPE

D.L. Fischer | G.K. Vignolo | M.G. Aldrighi | J.C. Fachinello | L.E.C. Antunes

38. IN VITRO DIRECT PLANT REGENERATION FROM LEAVES OF BLACKBERRY CULTIVAR ‘BOYSENBERRY’

Xiaomin Wang | Haiyan Li | Weilin Li | Wenlong Wu | Shuying Hu

39. EFFECTS OF POLYAMINES ON VEGETATIVE CHARACTERISTICS, GROWTH, FLOWERING AND YIELD OF STRAWBERRY (‘PAROS’ AND ‘SELVA’)

N. Movahed | S. Eshghi | E. Tafazoli | B. Jamali

40. ACCUMULATED LEAF NUMBER IN STRAWBERRY CULTIVARS GROWN IN A GREENHOUSE

H.F.C. Mendonça | A.L. Müller | I.F. Tazzo | E.O. Calvete

41. MANIPULATION OF CROPPING TIME BY CONTROLLING TIMING OF CANE ELONGATION IN PRIMOCANE-FRUITING RED RASPBERRY ‘HERITAGE’ IN JAPAN

H. Imanishi | T. Miyairi | M. Torii

42. EFFECT OF SHORTENING DAYLENGTH ON FLOWER BUD DIFFERENTIATION AND CROWN DEVELOPMENT IN ‘ELSANTA’ STRAWBERRY

K. Król | W. Lech

43. STUDY ON PHOTOSYNTHETIC CHARACTERISTICS OF BLUEBERRY IN GREENHOUSE

Xiaoyan Li | Wei Chen | Yadong Li

44. CHLOROPHYLL CONTENT IN STRAWBERRY LEAVES PRODUCED UNDER SHADING SCREENS IN GREENHOUSE

R.C. Costa | E.O. Calvete | J. Schons | F.H. Reginatto

45. EFFECT OF ABIOTIC ELICITORS ON ANTHOCYANIN PRODUCTION IN BERRY CELL SUSPENSIONS

46. COMPOSITIONAL CHANGES IN BLUEBERRY AND CRANBERRY FRUIT DURING RIPENING

C.F. Forney | W. Kalt | J.A. Jordan | M.R. Vinqvist-Tymchuk | S.A.E. Fillmore

47. ANTIOXIDANT RESPONSES OF STRAWBERRY PLANTS UNDER STRESS CONDITIONS

D. Neocleous | V. Ziogas | M. Vasilakakis

48. PHYSIOLOGICAL EFFECTS OF NITROGEN, PHOSPHORUS AND POTASSIUM ON BLUEBERRY ‘BLUECROP’

Xiaoyan Li | Jienan Wei | Yadong Li

49. INVESTIGATION OF FRUIT CHEMICAL COMPOSITION AND VEGETATIVE PROPAGATION OF CULTIVATED AND WILD ELDERBERRY

A. Matejicek | J. Kaplan | M. Vespalcova

50. A FRUIT RIPENING MODEL AND STORAGE CHARACTERISTICS OF BLUEBERRY

Wu Lin | Shen Lili | Li Xiangxin | Li Yadong | Zhang Zhidong

51. NARROW BAND REFLECTANCE MEASUREMENTS CAN BE USED TO ESTIMATE LEAF AREA INDEX, FLOWER NUMBER, FRUIT SET AND BERRY YIELD OF THE WILD BLUEBERRY (VACCINIUM ANGUSTIFOLIUM AIT.)

D.C. Percival | S. Sharpe | R. Maqbool | Q. Zaman

52. EFFECT OF CHILLING AND TWO PGRS ON SEED DORMANCY OF QARE-QAT BERRY (VACCINIUM ARCTOSTAPHYLOS L.)

S. Sedaghathoor

53. GENOTYPE, ENVIRONMENT AND CULTIVATION SYSTEMS: WHAT WILL BERRY PRODUCTION BE LIKE IN THE FUTURE?

C. Carlen | P. Crespo

54. PROGRESS AND CHALLENGES IN PRIMOCANE-FRUITING BLACKBERRY BREEDING AND CULTURAL MANAGEMENT

J.R. Clark | B.C. Strik | E. Thompson | C.E. Finn

55. AN OUTLINE ON RESEARCH AND UTILIZATION OF BLACKBERRY IN CHINA

Weilin Li | Wenlong Wu | Lianfei Lu

56. EFFECTED CULTURAL PRACTICES ON FRUIT QUALITY AND POLYPHENOLS CONTENT

S. Khanizadeh | M.T. Charles | C. Yu | S. Tao | L. Fan

57. SOIL BIOSOLARIZATION FOR STRAWBERRY CULTIVATION

58. LARGE PLOT DEMONSTRATIONS FOR METHYL BROMIDE ALTERNATIVES ON STRAWBERRY IN BAJA CALIFORNIA, MEXICO

J.M. López-Aranda | S. Urbina | G. Castellá | J.F. Estrada | M.A. Cotero

59. THE ANDALUSIAN NETWORK OF AGRARIAN EXPERIMENTS (RAEA) – THE CASE OF STRAWBERRY: AN OVERVIEW

J.J. Medina-Mínguez | J.M. López-Aranda | L. Miranda | P. Domínguez | C. Soria

60. RESPONSE OF COLD STORED DRIP-IRRIGATED STRAWBERRY TO ROOT TRIMMING AND TRANSPLANT CROWN DIAMETER

M.E. Ragab | A. Abo El-Yazied | M. Al-Sherif | N.M. Hassanin | H.H. Mohamed

61. EFFECTS OF CULTIVARS AND SUBSTRATES ON SOILLESS STRAWBERRY PRODUCTION IN CYPRUS

D. Neocleous | M. Vasilakakis

62. MATHEMATICAL MODELS, TABLES AND NOMOGRAMS TO ESTABLISH THE TECHNICAL OPTIMAL RATES (TOR) OF N, P₂O₅ AND K₂O IN STRAWBERRY, COMPARISON WITH SOME ERRONEOUS MODELS

G. Budoi

63. MATHEMATICAL MODELS, TABLES AND NOMOGRAMS CONCERNING PH VARIATION CAUSED BY CHANGES IN CONCENTRATION OF FESO₄.7H₂O IN AQUEOUS SOLUTIONS USED AS FOLIAR FEEDING

G. Budoi | E. Badea

64. THE EFFECT OF FOLIAR FERTILIZATION WITH CALCIUM ON QUALITY OF STRAWBERRY CULTIVARS ‘LUNA’ AND ‘ZANTA’

M. Bieniasz | M. Małodobry | E. Dziedzic

65. STRAWBERRY PLANT GROWTH AND DRY MATTER PARTITIONING AS INFLUENCED BY PLANTING DATE AND PLANT TYPE IN AN AUTUMN PRODUCTION SYSTEM

M.G. Palha | J.L. Campo | P.B. Oliveira

66. INTELLIGENT IRRIGATION OF LOWBUSH BLUEBERRIES IN ATLANTIC CANADA

J.P. Privé | L. Russell | P. Hildebrand | N. Boyd | D.C. Percival

67. EFFECTS OF EARLY PRUNING ON PRIMOCANES OF ‘MEIGUO 22’, ‘SHENGLI’ AND ‘VETEN’

Hanping Dai | Xiuwu Guo | Ge Song

68. EFFECT OF DIFFERENT TRANSPLANTING DATES AND RUNNER TYPES ON QUALITY AND YIELD OF ‘ELSANTA’ STRAWBERRY

G.T. Beyene | E. Kehoe | M. MacSiurtain | A. Hunter

69. ARBUSCULAR MYCORRHIZA ALLEVIATES SALINITY STRESS OF STRAWBERRY CULTIVARS UNDER SALINITY CONDITION

70. FACTORS ON INDUCED SYSTEMIC DISEASE RESISTANCE IN MYCORRHIZAL STRAWBERRY PLANTS

Y. Matsubara | Y. Li | T. Okada | M.A. Maya

71. OPTIMIZING A HIGH-DENSITY SOILLESS CULTURE SYSTEM FOR STRAWBERRY PRODUCTION

M.G. Palha | J.L. Campo | P.B. Oliveira

72. CHEMICAL ANALYSIS OF STRAWBERRY FRUIT GROWN IN A PROTECTED GREENHOUSE UNDER DIFFERENT SHADING SCREENS

R.C. Costa | E.O. Calvete | C.L. Bordignon Júnior | V. Francescatto | F.H. Reginatto

73. EFFECT OF PLANTING DENSITY AND PLASTIC SOIL MULCH ON STRAWBERRY PLANT DEVELOPMENT, YIELD AND FRUIT QUALITY

V. Laugale | L. Lepse | S. Strautina | I. Krasnova | D. Seglina

74. DIFFERENT TRAINING SYSTEMS FOR PHYSALIS PERUVIANA L. IN SOUTHERN BRAZIL

75. ACCUMULATION CHARACTERISTICS OF FRUIT WEIGHT, DIAMETER AND MAIN NUTRIENT COMPOSITION INDICES IN GROWING ‘BOYSENBERRY’ FRUIT

76. STRAWBERRY FRUIT PRODUCTION AND QUALITY UNDER CONVENTIONAL, INTEGRATED AND ORGANIC MANAGEMENT

P.A. Roussos | A. Triantafillidis | E. Kepolas

77. THE PHYLLOCHRON OF STRAWBERRY INTERCROPPED WITH FIG TREES IN A GREENHOUSE

78. EXTENDING STRAWBERRY PRODUCTION SEASON IN LATVIA

V. Laugale | J. Lepsis | S. Strautina

79. BLUEBERRY (V. CORYMBOSUM L.) POLLINATION WITH PRESENCE OF BOMBUS SP.

R.-A. Zajmi | S. Braha | M. Shehaj | A. Rexhepi

80. CIRCULAR MULTI-STORIED CULTURE MODE AMONG STRAWBERRY, MUSHROOM AND LEAF VEGETABLES IN GREENHOUSE

81. EFFECT OF TEMPERATURE AND HUMIDITY ON STRAWBERRY FIRMNESS AT TWO DIFFERENT SITES IN THE HUELVA REGION OF SPAIN

S. Pyrotis | L. Abayomi | D. Rees | J. Orchard

82. FIRST RESULTS OF BIODEGRADABLE MULCHING ON SMALL BERRY FRUITS

V. Girgenti | C. Peano | N.R. Giuggioli | E. Giraudo | S. Guerrini

83. MANAGING FARMING SYSTEMS, LANDSCAPES, PESTS AND PATHOGENS TO IMPROVE CONSUMER PERCEPTION OF BERRIES

M. Pritts

84. MANAGEMENT OF MONILINIA BLIGHT IN WILD BLUEBERRIES (VACCINIUM ANGUSTIFOLIUM AIT.): PRESENT CONCERNS AND FUTURE OPTIONS

D.C. Percival | E.F. Beaton

85. RASPBERRY LATENT VIRUS, A NEW REOVIRUS ISOLATED FROM CRUMBLY FRUITED RED RASPBERRY PLANTS

D. Quito | R.R. Martin

86. CONTROLLED ATMOSPHERE TEMPERATURE TREATMENT AS SUSTAINABLE ALTERNATIVE TO CONTROL STRAWBERRY TARSONEMID MITES AND PLANT PARASITIC NEMATODES IN STRAWBERRY PLANTS

G. van Kruistum | H. Hoek | J. Verschoor | L. Molendijk

87. THE PRESENCE, POPULATION STRUCTURE, AND POTENTIAL EFFECTS OF ENDOPHYTIC BACTERIA IN THE AMERICAN CRANBERRY

88. RASPBERRY BEETLE BYTURUS TOMENTOSUS: FLIGHT MONITORING WITH SEMIOCHEMICAL TRAPS IN SWITZERLAND

C.A. Baroffio

89. NEOFUSICOCCUM PARVUM, BLUEBERRY PATHOGEN IN ARGENTINA

E.R. Wright | A. Mandolesi | M.C. Rivera | B.A. Pérez

90. POPULATION FLUCTUATION OF SPIDER MITE TETRANYCHUS URTICAE IN DIFFERENT PRODUCTION SYSTEMS OF STRAWBERRY

J.P.T. Dias | J.D. Filho | E.L. Carmo | J.C. Simões | J.G. Pádua

91. DETECTION OF VIRUSES AND PHYTOPLASMA IN VACCINIUM SP. IN THE CZECH REPUBLIC

J. Spak | J. Pribylova | D. Kubelkova | K. Petrzik | V. Spakova

92. CHEMICAL AND NON-CHEMICAL ALTERNATIVES TO METHYL BROMIDE ON STRAWBERRY IN HUELVA (SPAIN): 2008-2010 RESULTS

93. SURVIVAL OF COLLETORICHUM ACUTATUM ON ALTERNATE HOSTS

P. Parikka | A. Lemmetty

94. LOGISTIC MODELS FOR ESTIMATING EPIDEMIC INCREASE RATES OF BLUEBERRY FOLIAR DISEASES, BASED ON METEOROLOGICAL AND LEAF SENESCENCE VARIABLES

95. IDENTIFICATION OF COLLETOTRICHUM ACUTATUM AND C. GLOEOSPORIOIDES ON STRAWBERRY IN EGYPT

96. ALLERGOMICS OF BERRY FRUITS

G. Marzban | F. Maghuly | M. Laimer | W. Hemmer | R. Nestby

97. ANTIOXIDANT ACTIVITY OF POLYPHENOL RICH FRUITS ON HUMAN ERYTHROCYTES

C. Widén | A. Ekholm | D. Piwowar-Zail | K. Rumpunen

98. PHENOLIC PHYTOCHEMICAL ANTIOXIDANTS IN BERRIES

C. Serrano | M.B. Sousa | M.J. Trigo | R. Bronze

99. NUTRACEUTICAL PROPERTIES IN ORGANIC STRAWBERRIES FROM SOUTH ITALY

F. Blando | R. Spirito | C. Gerardi | M. Durante | I. Nicoletti

100. PHENOLIC COMPOSITION OF ‘OSO GRANDE’ STRAWBERRY FRUITS IN DIFFERENT TIME OF HARVEST AND GROWN IN DIFFERENT PRODUCTION SYSTEMS

C.L. Bordignon Júnior | E.O. Calvete | A.A. Nienow | F.H. Reginatto

101. INFLUENCE OF GENOTYPE, LOCATION AND YEAR FACTORS ON QUALITY AND HEALTH PROMOTING COMPOUNDS OF RUBUS FRUITS

102. INFLUENCE OF TEMPERATURE ON BERRY QUALITY OF ‘GLEN AMPLE’ RED RASPBERRY

S.F. Remberg | A. Sønsteby | A.-B. Wold | O.M. Heide

103. ANTHOCYANINS IN BLUEBERRY CULTIVARS: EFFECT OF THE GROWING AREA

104. STUDY ON BIOLOGICAL VALUE CHANGES OF LATVIAN WILD CRANBERRIES DURING CONVECTIVE DRYING

K. Dorofejeva | T. Rakcejeva

105. MARKETING STRATEGY STUDY ON FRESH BLUEBERRIES IN CHINA

Li Limin | Wu Lin | Li Yishuang | Li Yadong

106. USE OF STRAWBERRY AS A TOOL TO DEVELOP A COMMUNITY WHERE PEOPLE CAN LIVE HARMONIOUSLY WITH THE FOREST IN THE NORTHEAST AREA OF THAILAND

P. Tongtraibhop | T. Thongthieng

107. THE RASPBERRY SUPPLY CHAIN AND ISSUES PERTAINING TO RASPBERRY PRODUCTION AREAS IN JAPAN

T. Miyairi | H. Imanishi