Articles
A DEVICE TO STUDY THE GROWTH OF FRUIT: DEPRESSIVE EFFECT OF THE NUTRIENT SOLUTION SUCROSE CONCENTRATION ON DETACHED TOMATO FRUIT GROWTH IN THE CELL EXPANSION PHASE
Article number
932_31
Pages
219 – 222
Language
English
Abstract
A new semi open device, named Casafruit, was designed to study tomato fruit growth.
This device was constituted of four boxes in which tomato fruits detached from the plant were grown with their pedicel immersed in a nutrient solution.
This solution was automatically replaced each day.
The fruit weight could be estimated frequently.
The fruits were placed in the device after an optimized disinfection treatment.
As the effect of sap sucrose concentration on fruit expansion is poorly investigated, this effect was studied with Casafruit. Tomato fruits taken from plants about twenty days after anthesis were placed in the device with a nutrient solution containing 5% sucrose.
After about eight days, sucrose concentration was increased to 15% in two boxes or maintained at 5% in the two other boxes.
The experiment was repeated five times.
No contamination occurred.
It was observed that fruit expansion rate decreased after the sucrose concentration was increased and the fruits became smaller than those grown at 5%.
Although the detached fruits were smaller than fruits grown on the plant, these results suggest that a high sap sucrose concentration in the period where tomato fruit expansion depends mainly on cell expansion has a depressive effect on growth.
The device Casafruit might be useful for diverse studies on fruit growth for instance to study the effect of sucrose in the cell multiplication phase.
This device was constituted of four boxes in which tomato fruits detached from the plant were grown with their pedicel immersed in a nutrient solution.
This solution was automatically replaced each day.
The fruit weight could be estimated frequently.
The fruits were placed in the device after an optimized disinfection treatment.
As the effect of sap sucrose concentration on fruit expansion is poorly investigated, this effect was studied with Casafruit. Tomato fruits taken from plants about twenty days after anthesis were placed in the device with a nutrient solution containing 5% sucrose.
After about eight days, sucrose concentration was increased to 15% in two boxes or maintained at 5% in the two other boxes.
The experiment was repeated five times.
No contamination occurred.
It was observed that fruit expansion rate decreased after the sucrose concentration was increased and the fruits became smaller than those grown at 5%.
Although the detached fruits were smaller than fruits grown on the plant, these results suggest that a high sap sucrose concentration in the period where tomato fruit expansion depends mainly on cell expansion has a depressive effect on growth.
The device Casafruit might be useful for diverse studies on fruit growth for instance to study the effect of sucrose in the cell multiplication phase.
Authors
P. Bussières, N. Bertin, C.E. Morris, C. Vigne, P. Orlando, C. Glaux, H. Floret, J. Bernadac, V. Sévénier, S. Pfeiffer
Keywords
in vitro culture, sterile medium
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