Articles
GIBBERELLIN METABOLISM DURING STEM ELONGATION STIMULATED BY HIGH TEMPERATURE IN LETTUCE
Article number
932_52
Pages
259 – 264
Language
English
Abstract
High temperatures induce bolting in lettuce.
Gibberellins (GAs) play an important role in the bolting of several plant species, including lettuce.
To reveal the role of GAs in this process, GA metabolic genes and endogenous GAs in early- and late-bolting cultivars were examined.
Quantitative reverse-transcription polymerase chain reaction indicated that LsGA3ox1, a GA 3-oxidase gene that metabolizes GA20 to GA1, was significantly up-regulated by higher temperatures compared to lower temperatures in lettuce cultivars.
Quantification of GA by liquid chromatography-mass spectrometry/mass spectrometry showed increased GA1 content in lettuce at higher temperatures.
The application of prohexadione-calcium to inhibit GA 3-oxidase expression reduced the stem length of lettuce grown at higher temperatures.
Levels of LsGA3ox1 expression and GA1 content in a late-bolting cultivar were lower than those in an early-bolting cultivar.
Our results suggest that LsGA3ox1 is a candidate for the gene responsible for increased GA1, but the mechanism(s) of GA metabolism and/or action may differ among cultivars with different bolting characteristics.
Gibberellins (GAs) play an important role in the bolting of several plant species, including lettuce.
To reveal the role of GAs in this process, GA metabolic genes and endogenous GAs in early- and late-bolting cultivars were examined.
Quantitative reverse-transcription polymerase chain reaction indicated that LsGA3ox1, a GA 3-oxidase gene that metabolizes GA20 to GA1, was significantly up-regulated by higher temperatures compared to lower temperatures in lettuce cultivars.
Quantification of GA by liquid chromatography-mass spectrometry/mass spectrometry showed increased GA1 content in lettuce at higher temperatures.
The application of prohexadione-calcium to inhibit GA 3-oxidase expression reduced the stem length of lettuce grown at higher temperatures.
Levels of LsGA3ox1 expression and GA1 content in a late-bolting cultivar were lower than those in an early-bolting cultivar.
Our results suggest that LsGA3ox1 is a candidate for the gene responsible for increased GA1, but the mechanism(s) of GA metabolism and/or action may differ among cultivars with different bolting characteristics.
Authors
M. Fukuda, S. Matsuo, K. Kikuchi, W. Mitsuhashi, T. Toyomasu, I. Honda
Keywords
bolting, GA 3-oxidase
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