Articles
BORON CONCENTRATION AFFECTS IN VITRO POLLEN GERMINATION AND TUBE GROWTH IN KIWIFRUIT (ACTINIDIA DELICIOSA) CULTIVAR ‘MATUA’
Article number
932_75
Pages
517 – 521
Language
English
Abstract
The effect of varying boron (B) concentrations on in vitro pollen germination and tube growth of Matua kiwifruit was evaluated.
Five concentrations of boric acid (H3BO3): 0, 25, 50, 100 and 500 mg L-1, were added to the germination media, prepared with 5% sucrose and 2% agar. Matua pollen was placed on the agar media and incubated in Petri dishes at 20°C for 48 h, with germination evaluated after 24 h.
Pollen incubated at concentrations of 25 and 50 mg L-1 boric acid showed 67.5 and 73.5% of pollen germination, respectively.
Significant reductions in pollen germination, 24.0 and 22.7%, were observed at B concentrations of 100 and 500 mg L-1, respectively.
In the media prepared without B, only 16.7% germination was observed.
After 48 h of incubation, in media prepared with 25 and 50 mg L-1 of boric acid, pollen tube lengths reached 53.2 and 66.7 µm, respectively, being significantly longer than those incubated in 100 and 500 mg L-1 B media (27.2 and 22.4 µm, respectively), and the 0 mg L-1 B media, where pollen tube length reached only 18.3 µm.
It can be concluded that boric acid concentrations 100 mg L-1 or higher in the growing media reduce Matua pollen germination and tube growth.
Five concentrations of boric acid (H3BO3): 0, 25, 50, 100 and 500 mg L-1, were added to the germination media, prepared with 5% sucrose and 2% agar. Matua pollen was placed on the agar media and incubated in Petri dishes at 20°C for 48 h, with germination evaluated after 24 h.
Pollen incubated at concentrations of 25 and 50 mg L-1 boric acid showed 67.5 and 73.5% of pollen germination, respectively.
Significant reductions in pollen germination, 24.0 and 22.7%, were observed at B concentrations of 100 and 500 mg L-1, respectively.
In the media prepared without B, only 16.7% germination was observed.
After 48 h of incubation, in media prepared with 25 and 50 mg L-1 of boric acid, pollen tube lengths reached 53.2 and 66.7 µm, respectively, being significantly longer than those incubated in 100 and 500 mg L-1 B media (27.2 and 22.4 µm, respectively), and the 0 mg L-1 B media, where pollen tube length reached only 18.3 µm.
It can be concluded that boric acid concentrations 100 mg L-1 or higher in the growing media reduce Matua pollen germination and tube growth.
Authors
C. Sotomayor, R. Ruiz, J. García-Huidobro
Keywords
pollen tube growth, boric acid, growing media, seed number
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