Articles
SPECIFIC IDENTIFICATION OF ATOXIGENIC STRAINS OF ASPERGILLUS FLAVUS ISOLATED FROM PISTACHIO ORCHARDS OF IRAN AND DETECTION OF AFLD, AFLR, AFLJ, AFLG GENES AND C3 REGION
Article number
963_15
Pages
95 – 97
Language
English
Abstract
Aspergillus flavus have genetically diverse populations in the field.
Different isolates produce either, neither or both aflatoxins and cyclopiazonic acid (CPA). The aim of this study was to investigate defects in the aflatoxin gene cluster in 17 non-aflatoxigenic A. flavus isolates collected from pistachio producing areas.
Genomic DNA was extracted using CTAB method.
The isolates were identified based on species-specific primers.
PCR assays using aflatoxin-gene-specific primers which have already been designed were used to group the functional or non-functional genes involved in aflatoxin production.
The PCR assays could show deletions in aflatoxin gene cluster for four genes of the aflatoxin biosynthetic pathway and one flanking region, aflD (nor1), aflR, aflJ(estA), aflG(avnA) and C3, respectively which was not rare in A. flavus isolates.
Different isolates produce either, neither or both aflatoxins and cyclopiazonic acid (CPA). The aim of this study was to investigate defects in the aflatoxin gene cluster in 17 non-aflatoxigenic A. flavus isolates collected from pistachio producing areas.
Genomic DNA was extracted using CTAB method.
The isolates were identified based on species-specific primers.
PCR assays using aflatoxin-gene-specific primers which have already been designed were used to group the functional or non-functional genes involved in aflatoxin production.
The PCR assays could show deletions in aflatoxin gene cluster for four genes of the aflatoxin biosynthetic pathway and one flanking region, aflD (nor1), aflR, aflJ(estA), aflG(avnA) and C3, respectively which was not rare in A. flavus isolates.
Authors
M. Moradi, H. Zamanizadeh, S.R. Fani, E. Sedaghati
Keywords
aflatoxin, gene cluster, nontoxigenic, molecular, PCR
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