Articles
EXPRESSION ANALYSIS OF KCS GENES POTENTIALLY INVOLVED IN CUTICULAR WAX PRODUCTION IN THE APPLE CULTIVAR ‘GEGESI ZÖLD’
Article number
981_30
Pages
205 – 208
Language
English
Abstract
The cuticle plays important roles in plants life, from controlling non-stomatal transpiration, through plant-pathogen interactions, to taking part in plant development.
In case of the model plant Arabidopsis thaliana, the biosynthetic pathways of cuticular lipids are well-studied, and at least partially understood.
It is known that an elongation complex outside the chloroplast extends plastidial C16 or C18 chain length fatty acids.
The resulting very long chain fatty acid will be modified to different lipoid compounds that finally load on the outer surface of the cuticle.
A battery of enzymes has been identified performing chain elongation, modifications and transport of the lipid molecules.
In our experiments, we wished to identify some KCS gene homologs in apple, potentially involved in these processes, and analyze expression of the candidate genes in different fruit tissues.
Based on data from the literature dealing with the Arabidopsis model we performed in silico analysis of apple EST as well as genome sequences and found candidate KCS genes that could be related to wax production.
Subsequent RT-PCR analysis of most of these sequences showed them to be expressed in the fruit, furthermore in some cases the expression was peel-specific.
This makes those sequences likely candidates for apple genes involved in fruit cuticular wax production.
In case of the model plant Arabidopsis thaliana, the biosynthetic pathways of cuticular lipids are well-studied, and at least partially understood.
It is known that an elongation complex outside the chloroplast extends plastidial C16 or C18 chain length fatty acids.
The resulting very long chain fatty acid will be modified to different lipoid compounds that finally load on the outer surface of the cuticle.
A battery of enzymes has been identified performing chain elongation, modifications and transport of the lipid molecules.
In our experiments, we wished to identify some KCS gene homologs in apple, potentially involved in these processes, and analyze expression of the candidate genes in different fruit tissues.
Based on data from the literature dealing with the Arabidopsis model we performed in silico analysis of apple EST as well as genome sequences and found candidate KCS genes that could be related to wax production.
Subsequent RT-PCR analysis of most of these sequences showed them to be expressed in the fruit, furthermore in some cases the expression was peel-specific.
This makes those sequences likely candidates for apple genes involved in fruit cuticular wax production.
Publication
Authors
ZS. Albert, B. Ivanics, A. Molnár, CS. Deák, A. Miskó, I. Papp, M. Tóth
Keywords
apple, gene expression, wax, cuticle
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