Articles
MICROPROPAGATION OF TWO BLUE HONEYSUCKLE CULTIVARS RELEASED IN ROMANIA
Article number
981_72
Pages
453 – 459
Language
English
Abstract
Lonicera caerulea is a honeysuckle native throughout the cool temperature Northern Hemisphere.
It was introduced in Romania for research purposes in 1988 and the interest for this species increased because of its fruit biochemical content and economic value.
We have developed in vitro propagation biotechnology for rapid shoot multiplication of two honeysuckles (Lonicera caerulea L. var. kamtschatica Sevast.) cultivars Loni and Cera, released in Romania in 2003. These cultivars were highlighted to be approved and extended in production for both dessert and fruit processing industries.
Cultures were started by using actively growing shoots from field grown shrubs.
Buds were surface sterilized with ethanol and calcium hypochlorite.
The best initiation medium for both Lonicera cultivars was Quoirin & Lepoivre (QL) basal salt mixture and Linsmaier & Skoog (LS) vitamins, containing 0.1 mg/L GA3, and 1mg/L IBA. Multiplication rate varied depending on the cultivar and growth regulators concentrations.
The highest multiplication rate was obtained using 1 mg/L BAP and 0.05 mg/L GA3. Excised axillary shoots were rooted in vitro in presence of IBA. Microshoots rooted differently depending on the treatment and cultivar.
In case of Loni cultivar 89.6% rooting was achieved by treatment with 1 mg/L IBA and 162 mg/L PG when Murashige & Skoog (MS) basal nutrient medium was used.
The plantlets were subsequently transferred into different substrates for acclimatization.
This research showed that two Lonicera cultivars responded differently in culture. Loni cultivar was more prolific than Cera in the conditions tested.
It was introduced in Romania for research purposes in 1988 and the interest for this species increased because of its fruit biochemical content and economic value.
We have developed in vitro propagation biotechnology for rapid shoot multiplication of two honeysuckles (Lonicera caerulea L. var. kamtschatica Sevast.) cultivars Loni and Cera, released in Romania in 2003. These cultivars were highlighted to be approved and extended in production for both dessert and fruit processing industries.
Cultures were started by using actively growing shoots from field grown shrubs.
Buds were surface sterilized with ethanol and calcium hypochlorite.
The best initiation medium for both Lonicera cultivars was Quoirin & Lepoivre (QL) basal salt mixture and Linsmaier & Skoog (LS) vitamins, containing 0.1 mg/L GA3, and 1mg/L IBA. Multiplication rate varied depending on the cultivar and growth regulators concentrations.
The highest multiplication rate was obtained using 1 mg/L BAP and 0.05 mg/L GA3. Excised axillary shoots were rooted in vitro in presence of IBA. Microshoots rooted differently depending on the treatment and cultivar.
In case of Loni cultivar 89.6% rooting was achieved by treatment with 1 mg/L IBA and 162 mg/L PG when Murashige & Skoog (MS) basal nutrient medium was used.
The plantlets were subsequently transferred into different substrates for acclimatization.
This research showed that two Lonicera cultivars responded differently in culture. Loni cultivar was more prolific than Cera in the conditions tested.
Publication
Authors
V. Isac, GH. Mladin
Keywords
Lonicera caerulea, culture initiation, media, micropropagation, rhizogenesis, 6-benzylaminopurine
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