Articles
CHONDROITIN SULFATE CAN BE A NEW PLANT GROWTH REGULATOR FOR CYMBIDIUM MICROPROPAGATION©
Article number
1014_99
Pages
449 – 455
Language
English
Abstract
Chondroitin sulfate is unbranched polysaccharides of variable length containing two alternating monosaccharides: D-glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc). It is a major component of extracellular matrix, and is important in maintaining the structural integrity of the tissue.
It is widely used as a material for food ingredient, cosmetics, and medicine.
The present study was conducted to investigate the effect of chondroitin sulfate on in vitro regulation of protocorm-like bodies (PLB) of Cymbidium insigne under different quality of light (white fluorescent tube, green LED, and red LED). Recently, LEDs have drawn considerable interest as an alternative light source for in vitro propagation.
New PLB and shoots were successfully regenerated on modified Murashige and Skoog medium supplemented with chondroitin sulfate under different lights.
The highest PLB formation and shoot formation rate (80%) were found amongst explants cultured on medium supplemented with 0.1 mgL-1 chondroitin sulfate under white fluorescent light.
The chondroitin sulfate at every concentration enhanced the growth of PLB formation rate (100%) and the shoot formation rate 90% found at 1 and 10 mgL-1 chondroitin sulfate under green LED. The highest percentages of PLB formation was 100% and shoot formation was 90% obtained from the medium supplemented with 0.1 mgL-1 chondroitin sulfate under red LED, but the color of new PLB and shoots was not green.
These results indicated that chondroitin sulfate would work as a plant growth regulator which enhances both PLB and shoot formation and this newly developed light source could be used as an energy efficient light source for the propagation of Cymbidium PLB in vitro and comparatively green light showed best formation of PLB and shoot.
It is widely used as a material for food ingredient, cosmetics, and medicine.
The present study was conducted to investigate the effect of chondroitin sulfate on in vitro regulation of protocorm-like bodies (PLB) of Cymbidium insigne under different quality of light (white fluorescent tube, green LED, and red LED). Recently, LEDs have drawn considerable interest as an alternative light source for in vitro propagation.
New PLB and shoots were successfully regenerated on modified Murashige and Skoog medium supplemented with chondroitin sulfate under different lights.
The highest PLB formation and shoot formation rate (80%) were found amongst explants cultured on medium supplemented with 0.1 mgL-1 chondroitin sulfate under white fluorescent light.
The chondroitin sulfate at every concentration enhanced the growth of PLB formation rate (100%) and the shoot formation rate 90% found at 1 and 10 mgL-1 chondroitin sulfate under green LED. The highest percentages of PLB formation was 100% and shoot formation was 90% obtained from the medium supplemented with 0.1 mgL-1 chondroitin sulfate under red LED, but the color of new PLB and shoots was not green.
These results indicated that chondroitin sulfate would work as a plant growth regulator which enhances both PLB and shoot formation and this newly developed light source could be used as an energy efficient light source for the propagation of Cymbidium PLB in vitro and comparatively green light showed best formation of PLB and shoot.
Authors
Syeda Jabun Nahar, Kazuhiko Shimasaki, Syed Mostafizul Haque
Keywords
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