DESCRIPTION OF EXPRESSED PROTEINS ASSOCIATED TO THE SELF-INCOMPATIBLE AND SELF-COMPATIBLE RESPONSE IN ALMOND [PRUNUS DULCIS (MILLER) D.A. WEBB]

In almond, as in other Prunus species, the self-incompatibility mechanism is of the gametophytic type.
Gametophytic self-incompatibility (GSI) is controlled by a highly polymorphic locus named S, which includes two tightly linked genes expressed in the pistil and in the pollen.
However, the exact function of this mechanism is still unknown.
To explore the factors involved in this type of response, a quantitative differential study of protein expression was performed using 2D-DIGE and HPLC-MS/MS techniques.
In addition, an exhaustive design using multiple compatible and incompatible crosses was carried out.
Pollen and pistil tissue from accessions of the self-incompatible almond cultivars ‘Ferragnès’, ‘Mono’, ‘Titan’ and ‘Primorskyi’, and of the homozygous self-compatible almond selection ‘A2198’ were used in this study.
According to the results, 24 spots were differentially expressed after comparison between the compatible and incompatible responses.
These results provide the first protein profile to progress in the understanding of self-incompatibility in almond.

VI International Symposium on Almonds and Pistachios

P.J. Martínez-García, F. Dicenta, E. Ortega

Prunus dulcis, self-incompatibility, differential protein expression, 2D-DIGE, HPLC-MS/MS

https://doi.org/10.17660/ActaHortic.2014.1028.22