Articles
IN VITRO PROPAGATION OF BARKERIA SHOEMAKER HALB.
Article number
1083_21
Pages
181 – 188
Language
English
Abstract
This research established the optimal conditions for in vitro seed germination, development and acclimatization of Barkeria shoemaker seedlings.
This species is a small orchid endemic to Mexico.
It has pink-pale lilac flowers, white or pink-lilac labellum, with intense magenta veins along the labellum, its column is yellow-greenish dotted in purple.
Seeds were germinated in Murashige and Skoog (MS) and Knudson C (KC) culture media.
Six concentrations of sucrose (15, 20, 25, 30, 35 and 40 g L-1) were evaluated during the multiplication stage.
The effect of naphthaleneacetic acid (NAA) (0.0, 0.2, 0.5 and 1.0 mg L-1) and benzyladenine (BA) (0.0, 0.5 and 1.0 mg L-1) was studied in another trial, in which a factorial arrangement was established in a completely randomized design with six replications.
Seed germination began 15 days after sowing (DAS) in MS medium and 20 DAS in KC medium; in MS medium, the seedlings were 41% larger (8.35 mm) and weighed twice as much as those in KC medium.
The KC medium stimulated the number of roots per plant (0.8) and their length (5 cm). During the seedling multiplication stage, the highest number of leaves (5.38) was obtained with 15 and 20 g L-1 sucrose, at 90 DAS; on the other hand, with 35 g L-1 sucrose the number of leaves per plant decreased by 34%. The BA and NAA concentrations did not affect the response variables compared to the control treatment; however, with higher levels (1.0 mg L-1), there was a tendency for the values of the recorded variables to increase.
In sphagnum turbe, 95% of the plants transferred to the greenhouse were successfully acclimatized.
Two months after the transfer, 20% of the seedlings flowered, which gives this species great ornamental value as a miniature plant.
This species is a small orchid endemic to Mexico.
It has pink-pale lilac flowers, white or pink-lilac labellum, with intense magenta veins along the labellum, its column is yellow-greenish dotted in purple.
Seeds were germinated in Murashige and Skoog (MS) and Knudson C (KC) culture media.
Six concentrations of sucrose (15, 20, 25, 30, 35 and 40 g L-1) were evaluated during the multiplication stage.
The effect of naphthaleneacetic acid (NAA) (0.0, 0.2, 0.5 and 1.0 mg L-1) and benzyladenine (BA) (0.0, 0.5 and 1.0 mg L-1) was studied in another trial, in which a factorial arrangement was established in a completely randomized design with six replications.
Seed germination began 15 days after sowing (DAS) in MS medium and 20 DAS in KC medium; in MS medium, the seedlings were 41% larger (8.35 mm) and weighed twice as much as those in KC medium.
The KC medium stimulated the number of roots per plant (0.8) and their length (5 cm). During the seedling multiplication stage, the highest number of leaves (5.38) was obtained with 15 and 20 g L-1 sucrose, at 90 DAS; on the other hand, with 35 g L-1 sucrose the number of leaves per plant decreased by 34%. The BA and NAA concentrations did not affect the response variables compared to the control treatment; however, with higher levels (1.0 mg L-1), there was a tendency for the values of the recorded variables to increase.
In sphagnum turbe, 95% of the plants transferred to the greenhouse were successfully acclimatized.
Two months after the transfer, 20% of the seedlings flowered, which gives this species great ornamental value as a miniature plant.
Authors
E. Paniagua-Jasso, M.E. Pedraza-Santos, N. Gutiérrez-Rangel, L.M. Tapia-Vargas
Keywords
asymbiotic germination, endangered orchid, in vitro flowering micropropagation, Orchidaceae
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