Articles
THE INFLUENCE OF CYTOKININ AND AUXIN TYPES AND THEIR CONCENTRATION ON THE PROLIFERATION AND ROOTING OF VIBURNUM TRELEASEI SEEDLING EXPLANTS
Article number
1083_39
Pages
311 – 318
Language
English
Abstract
Recent demands on roadsides plantations and landscape restoration with native species along with species conservation, require huge amounts of individuals yet conserving their genetic diversity.
This work aimed the establishment of Viburnum treleasei in vitro cultures from seedlings and the optimization of the in vitro shoots proliferation and rooting stages.
With this purpose 500 embryos were isolated and inoculated on WPM (Woody Plant Medium). The seedlings nodal explants were then inoculated on WPM supplemented with 1.14, 4.56 and 22.81 µM of zeatin, 1.23, 4.92 and 24.6 µM of 2iP (N6[2-isopentenyl]adenine) or 1.11, 4.44 and 22.2 µM of BA (6-benzyladenine). After eight weeks, the nodal segments were subcultured in the same media formulations.
Before root induction the plantlets were subcultured 8 weeks on WPM without growth regulators.
Root induction was carried out by transferring plantlets (>1 cm) to a WPM supplemented with 0, 1.34, 5.37 and 10.74 µM of NAA (α-naphthaleneacetic acid), 0, 1.23, 4.92 and 9.84 µM of IBA (3-indolebutyric acid), or 0, 1.43, 5.71 and 11.42 µM of IAA (3-indolacetic acid). The best proliferative responses of shoots in the two subcultures were obtained in the medium containing 22.81 µM zeatin.
A significant increase of rooting percentages was only achieved on the media supplemented with NAA and after eight weeks the best rooting induction was achieved with 5.37 and 10.74 µM NAA.
This work aimed the establishment of Viburnum treleasei in vitro cultures from seedlings and the optimization of the in vitro shoots proliferation and rooting stages.
With this purpose 500 embryos were isolated and inoculated on WPM (Woody Plant Medium). The seedlings nodal explants were then inoculated on WPM supplemented with 1.14, 4.56 and 22.81 µM of zeatin, 1.23, 4.92 and 24.6 µM of 2iP (N6[2-isopentenyl]adenine) or 1.11, 4.44 and 22.2 µM of BA (6-benzyladenine). After eight weeks, the nodal segments were subcultured in the same media formulations.
Before root induction the plantlets were subcultured 8 weeks on WPM without growth regulators.
Root induction was carried out by transferring plantlets (>1 cm) to a WPM supplemented with 0, 1.34, 5.37 and 10.74 µM of NAA (α-naphthaleneacetic acid), 0, 1.23, 4.92 and 9.84 µM of IBA (3-indolebutyric acid), or 0, 1.43, 5.71 and 11.42 µM of IAA (3-indolacetic acid). The best proliferative responses of shoots in the two subcultures were obtained in the medium containing 22.81 µM zeatin.
A significant increase of rooting percentages was only achieved on the media supplemented with NAA and after eight weeks the best rooting induction was achieved with 5.37 and 10.74 µM NAA.
Authors
M.J. Pereira, T. Eleutério, J.M. Canhoto
Keywords
3-indolacetic acid, 3-indolebutyric acid, 6-benzyladenine, α-naphthalene-acetic acid, embryos, in vitro, N6[2-isopentenyl]adenine, zeatin
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