EFFECT OF KINETIN ON THE ELONGATION OF ADVENTITIOUS SHOOTS REGENERATED IN VITRO FROM LIGULATE FLORETS IN CHRYSANTHEMUM × GRANDIFLORUM RAMAT. KITAM.

Ligulate florets are precious explants in the breeding of new chrysanthemum cultivars, facilitating the production of a considerable number of adventitious shoots and the separation of the components of chimeras.
However, a substantial part of the shoots regenerated from floral explants do not reach the length allowing them to be easily cut off from the explant, making the division while multiplication and transferring onto the rooting medium.
The aim of the research was to determine the effect of KIN on the stimulation of the elongation growth of the shoots regenerated from ligulate florets of two cultivars of chrysanthemums ‘Breeze White’ and ‘Capitola’. The MS medium used was modified by increasing the content of calcium and iron by half, increasing the concentration of thiamine to 0.4 mg/L and decreasing the content of sucrose to 10 g/L. The ligulate florets transversely cut into half were horizontally inoculated with the abaxial side on the medium with 3 mg/L BAP and 0.5 mg/L IAA. Six weeks after the start of the culture, half of the explants were transferred onto the medium in which BAP was replaced with 1 mg/L KIN. The length of the shoots regenerated from ligulate florets on the medium with KIN added was almost three-fold higher than the length of the shoots formed from the ligulate florets present throughout the culture period on the medium with 3 mg/L BAP and 0.5 mg/L IAA. The ‘Breeze White’ shoots were almost twice as long as the shoots in ‘Capitola’. The percentage share of the explants undertaking the shoot regeneration was more than twice as high in ‘Capitola’, and it accounted for more than 50%.

VIII International Symposium on In Vitro Culture and Horticultural Breeding

M. Jerzy, M. Zalewska, A. Tymoszuk

chrysanthemum, growth regulators, petal, micropropagation

https://doi.org/10.17660/ActaHortic.2015.1083.77