Articles
EFFECT OF CULTURE MEDIUM AND 6-BENZYLADENINE CONCENTRATION ON THE MICROPROPAGATION OF VITIS LABRUSCA × VITIS ROTUNDIFOLIA HYBRIDS
Article number
1083_47
Pages
375 – 382
Language
English
Abstract
The success of micropropagation is mainly dependent on the choice of culture medium and growth regulators that control morphogenesis in vitro.
The objective of this study was to evaluate the effect of three culture media, C2D, half-strength MS and QL, and the effect of different concentrations of 6-benzyladenine (0, 1.0, 2.5 and 5.0 µM) on three subcultures of three grapevine hybrids from nodal segments.
The hybrids were IC125 (V. labrusca Isabel × V. rotundifolia Carlos), IB481 (V. Labrusca Isabel × V. Rotundifolia Bountiful) and IM628 (V. labrusca Isabel × V. rotundifolia Magnolia). Subcultures were performed every 40 days.
All of the cultures were exposed to a photoperiod of 16-h light/8-h dark.
The experiments were performed using a completely randomized design with a split-plot arrangement, and a subculture was treated as a subplot with four replications.
The means were compared using Tukeys test and regression analysis.
The C2D medium showed the least favorable result for all variables in the first subculture of IB481 and IM628 and was therefore not used for the second and third subcultures.
The MS/2 medium showed the best results with respect to number of shoots, number of leaves, height of shoots and rooting in the three subcultures of all of the hybrids; however, IB481 and IM628 showed less callus formation.
A BA concentration of 5.0 µM promoted the highest number of shoots and leaves; however, a reduction in height was observed.
Rooting decreased and callus formation increased gradually with increasing concentrations of BA; these effects were observed for all of the hybrids.
For in vitro growth of V. labrusca × V. rotundifolia hybrids, culture in half-strength MS medium supplemented with 5.0 µM BA is recommended.
The objective of this study was to evaluate the effect of three culture media, C2D, half-strength MS and QL, and the effect of different concentrations of 6-benzyladenine (0, 1.0, 2.5 and 5.0 µM) on three subcultures of three grapevine hybrids from nodal segments.
The hybrids were IC125 (V. labrusca Isabel × V. rotundifolia Carlos), IB481 (V. Labrusca Isabel × V. Rotundifolia Bountiful) and IM628 (V. labrusca Isabel × V. rotundifolia Magnolia). Subcultures were performed every 40 days.
All of the cultures were exposed to a photoperiod of 16-h light/8-h dark.
The experiments were performed using a completely randomized design with a split-plot arrangement, and a subculture was treated as a subplot with four replications.
The means were compared using Tukeys test and regression analysis.
The C2D medium showed the least favorable result for all variables in the first subculture of IB481 and IM628 and was therefore not used for the second and third subcultures.
The MS/2 medium showed the best results with respect to number of shoots, number of leaves, height of shoots and rooting in the three subcultures of all of the hybrids; however, IB481 and IM628 showed less callus formation.
A BA concentration of 5.0 µM promoted the highest number of shoots and leaves; however, a reduction in height was observed.
Rooting decreased and callus formation increased gradually with increasing concentrations of BA; these effects were observed for all of the hybrids.
For in vitro growth of V. labrusca × V. rotundifolia hybrids, culture in half-strength MS medium supplemented with 5.0 µM BA is recommended.
Authors
V. Reinhart, L.A. Biasi
Keywords
cytokinin, grape, muscadine
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