Articles
EXOGENOUS POLYAMINES AND ETHYLENE ON AECHMEA DISTICHANTHA L. (BROMELIACEAE) MICROPROPAGATION
Article number
1083_28
Pages
235 – 239
Language
English
Abstract
The aim of the research was to study the effects of exogenous polyamines putrescine, spermidine and spermine and ethylene on in vitro morphogenesis of Aechmea distichantha (Bromeliaceae). The plants obtained in vitro by shoots division were transferred to MS growth regulators free, MS + 10 µmol Put, MS + 10 µmol Spd, MS + 10 µmol Spm, MS + 10 mg/L ethylene and MS + 20 mg/L ethylene.
Plants were harvest after 0, 15, 30, 45 and 60 days of culture.
Number of shoots and roots and endogenous concentrations of polyamines Put, Spd and Spm, protein and peroxidase activity in leaves were evaluated.
Spd stimulated shoots formation on A. distichantha. All treatments had deleterious effect on rhizogenesis.
Plants treated with polyamines had higher proteins levels when compared to ethylene indicating the growing and development of explants.
Ethylene treatment had no effect on polyamines levels.
Thus, the biosynthetic routes of ethylene and polyamines may not compete for the common precursor S-adenosylmethionine.
Plants were harvest after 0, 15, 30, 45 and 60 days of culture.
Number of shoots and roots and endogenous concentrations of polyamines Put, Spd and Spm, protein and peroxidase activity in leaves were evaluated.
Spd stimulated shoots formation on A. distichantha. All treatments had deleterious effect on rhizogenesis.
Plants treated with polyamines had higher proteins levels when compared to ethylene indicating the growing and development of explants.
Ethylene treatment had no effect on polyamines levels.
Thus, the biosynthetic routes of ethylene and polyamines may not compete for the common precursor S-adenosylmethionine.
Authors
A.R. Tavares, S. Kanashiro, G.P.P. Lima
Keywords
Bromeliaceae, ethylene, peroxidase, polyamines, protein
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