Articles
EFFECT OF PHOSPHORUS ON IN VITRO GROWTH AND DEVELOPMENT OF BROMELIAD AECHMEA BLANCHETIANA
Article number
1083_29
Pages
241 – 248
Language
English
Abstract
The influence of different phosphorus concentrations in ionic balanced Murashige and Skoog medium on growth of Bromeliad Aechmea blanchetiana was studied.
The plants of A. blanchetiana were obtained through in vitro sowing and were transferred to MS liquid medium (Murashige and Skoog, 1962), modified with 0.15, 0.30, 0.60, 1.25 or 2.50 mM P (as phosphate). Constant concentrations of all nutrients of MS medium were kept by ionic balance, prior to the adjustment of pH to 5.8. The results showed that phosphorus influenced the growth of seedlings of A. blanchetiana cultured in MS medium, whereas the concentration of 2.5 mM of phosphate in modified MS medium provided the highest production of fresh and dry weight in both shoot and root system.
Variance analysis of regression proved that the contents of variables nitrogen and potassium content in leaves of A. blanchetiana showed no significant trend with increasing phosphate concentration in modified MS medium.
Phosphorus and sulfur content were significant only in the linear regression (α≤0.05) and each mM phosphate added to MS medium led to an increase of 0.98 and 0.16 mg of phosphorus and sulfur in leaves, respectively.
The plants of A. blanchetiana were obtained through in vitro sowing and were transferred to MS liquid medium (Murashige and Skoog, 1962), modified with 0.15, 0.30, 0.60, 1.25 or 2.50 mM P (as phosphate). Constant concentrations of all nutrients of MS medium were kept by ionic balance, prior to the adjustment of pH to 5.8. The results showed that phosphorus influenced the growth of seedlings of A. blanchetiana cultured in MS medium, whereas the concentration of 2.5 mM of phosphate in modified MS medium provided the highest production of fresh and dry weight in both shoot and root system.
Variance analysis of regression proved that the contents of variables nitrogen and potassium content in leaves of A. blanchetiana showed no significant trend with increasing phosphate concentration in modified MS medium.
Phosphorus and sulfur content were significant only in the linear regression (α≤0.05) and each mM phosphate added to MS medium led to an increase of 0.98 and 0.16 mg of phosphorus and sulfur in leaves, respectively.
Authors
A.R. Tavares, S. Kanashiro, R.C.S. Ribeiro, A.N. Gonçalves, T. Jocys
Keywords
Bromeliaceae, ionic balance, micropropagation, phosphorus, plant nutrition
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