Articles
Cloning and characteristics of flavonol synthase promoter from Malus crabapple
Article number
1106_19
Pages
123 – 130
Language
English
Abstract
A 1.8-kb 5RSQUO-upstream sequence of flavonol synthase (McFLS) from LSQUOever-red-leafRSQUO color types of Malus crabapple cultivar LSQUORoyaltyRSQUO was isolated.
The PLACE database (a database of motifs found in plant cis-acting regulatory DNA elements) analysis showed that there were numerous functional elements in the McFLS promoter.
Many light-responsive elements that indicated the expression of McFLS had a close relationship with light induction was discovered.
Abundant auxin and gibberellic acid (GA) responsive elements suggested that McFLS mainly expressed in the early development stage.
Many stress-responsive elements were consistent with the functional characteristics of flavonols in induction and stress-resistance reactions.
The expression level analysis verificated the role of cis-element in McFLS promoter.
To conclude, the McFLS promoter has potential research significance to explore the function of McFLS.
The PLACE database (a database of motifs found in plant cis-acting regulatory DNA elements) analysis showed that there were numerous functional elements in the McFLS promoter.
Many light-responsive elements that indicated the expression of McFLS had a close relationship with light induction was discovered.
Abundant auxin and gibberellic acid (GA) responsive elements suggested that McFLS mainly expressed in the early development stage.
Many stress-responsive elements were consistent with the functional characteristics of flavonols in induction and stress-resistance reactions.
The expression level analysis verificated the role of cis-element in McFLS promoter.
To conclude, the McFLS promoter has potential research significance to explore the function of McFLS.
Authors
J. Zhang, J. Tian, T. Song, Y. Yao
Keywords
FLS, promoter, Malus crabapple, cis-element
Online Articles (36)