Articles
MOTILITY AND CHEMOTAXIS OF ERWINIA AMYLOVORA
Article number
117_22
Pages
125 – 130
Language
Abstract
Chemotaxis of Erwinia amylovora is temperature and pH dependent with an optimum temperature range of 20–28 C and pH 6–8. An incubation period of 30 minutes and a cell population not greater than 4 x 107 cells/ml are optimal for chemotaxis studies.
A medium consisting of 10-3M ethylene diaminetetraacetic acid, 10-3M mannitol, 10-2M MgCl2 and 10-2M phosphate buffer at pH 7 was established for assays.
Using these assay conditions, E. amylovora exhibits positive chemotaxis to apple nectar, to one amino acid, aspartate, to the organic acids fumarate, malate, maleate, malonate, oxaloacetate, and succinate, but is not to any of the sugars tested.
All attractants are dicarboxylic acids and responses are uniformly inhibited by malate suggesting a single chemoreceptor site for all the attractants.
A medium consisting of 10-3M ethylene diaminetetraacetic acid, 10-3M mannitol, 10-2M MgCl2 and 10-2M phosphate buffer at pH 7 was established for assays.
Using these assay conditions, E. amylovora exhibits positive chemotaxis to apple nectar, to one amino acid, aspartate, to the organic acids fumarate, malate, maleate, malonate, oxaloacetate, and succinate, but is not to any of the sugars tested.
All attractants are dicarboxylic acids and responses are uniformly inhibited by malate suggesting a single chemoreceptor site for all the attractants.
Publication
Authors
A.K. Raymundo, S.M. Ries
Keywords
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