Home > International Symposia on Tropical and Temperate Horticulture – ISTTH2016 > Strategies for maintaining and increasing throughput of in vitro cultures of sugarcane

Articles

Strategies for maintaining and increasing throughput of in vitro cultures of sugarcane

Article number

1205_95

Pages

763 – 768

Language

English

Abstract

Simple in vitro protocols were developed or improved for in vitro manipulations of sugarcane (Saccharum species hybrids) to free up resources, deal with back-logs and increase throughput in research and commercial laboratories.
These include: (1) in vitro germination of field-derived axillary buds for meristem isolation; (2) a ‘common’ medium for rapid shoot multiplication resulting from single genetic modification events; (3) shoot storage for 12 months; (4) ‘holding-back’ in vitro plantlets before acclimation.
The novel strategies have proven reliable and effective in managing changing demands on resources and priorities.

Publication

International Symposia on Tropical and Temperate Horticulture – ISTTH2016

Authors

S.J. Snyman, M. Banasiak, P. Mhlanga, E. Mupanehari, M.P. Watt

Keywords

acclimation, in vitro sett germination, medium-term storage, shoot multiplication

Full text

https://doi.org/10.17660/ActaHortic.2018.1205.95

Groups involved

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