Articles
Genetic association among Indian melon accessions, including Agrestis and Momordica landraces
Article number
1255_8
Pages
41 – 46
Language
English
Abstract
Genetic diversity analysis of 70 melon accessions collected from various agro-ecological regions of the world viz., India, Afghanistan, USA and Israel was performed using 30 SSR markers.
The four markers, CMAG59, CMTCN38, CMMS3-1 and EMS178 revealed up to 4 alleles.
Seven SSR markers revealed specific/unique alleles and identified 23 genotypes.
Across the genotypes, maximum number of alleles (58) was detected in IC-274034 and the percentage of polymorphic markers was maximum (86.9) in PAUS-19. In the present study, average observed heterozygosity (53%) in melon germplasm collection was higher as compared to the average expected heterozygosity (48%). The cluster analysis illustrating genetic relationship, classified genotypes into three major clusters, Cluster I, Cluster II, and Cluster III. The similarity coefficient estimated by dice similarity coefficient ranged from 0.34 to 0.91. Clustering pattern was independent of horticultural groups and geographic origin.
High PIC values, number of alleles and observed heterozygosity frequency, explained relatively high genetic diversity present among melon accessions.
The four markers, CMAG59, CMTCN38, CMMS3-1 and EMS178 revealed up to 4 alleles.
Seven SSR markers revealed specific/unique alleles and identified 23 genotypes.
Across the genotypes, maximum number of alleles (58) was detected in IC-274034 and the percentage of polymorphic markers was maximum (86.9) in PAUS-19. In the present study, average observed heterozygosity (53%) in melon germplasm collection was higher as compared to the average expected heterozygosity (48%). The cluster analysis illustrating genetic relationship, classified genotypes into three major clusters, Cluster I, Cluster II, and Cluster III. The similarity coefficient estimated by dice similarity coefficient ranged from 0.34 to 0.91. Clustering pattern was independent of horticultural groups and geographic origin.
High PIC values, number of alleles and observed heterozygosity frequency, explained relatively high genetic diversity present among melon accessions.
Authors
S.P. Sharma, D. Singh, G. Singh, N.K. Sarao, V.K. Vashisht
Keywords
cluster analysis, genetic variation, molecular markers, muskmelon, PIC
Groups involved
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Tropical and Subtropical Fruit and Nuts
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Commission Banana
- Division Horticulture for Development
- Division Precision Horticulture and Engineering
- Division Plant Genetic Resources, Breeding and Biotechnology
- Commission Cultivar Registration
- Division Plant-Environment Interactions in Field Systems
- Division Postharvest and Quality Assurance
- Division Greenhouse and Indoor Production Horticulture
- Division Landscape and Urban Horticulture
- Division Horticulture for Human Health
- Division Temperate Tree Nuts
- Commission Agroecology and Organic Farming Systems
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