Articles
Morphogenetic potential of grape explants at initiation stage of in vitro culture during the active plant growth and dormancy periods
Article number
1324_17
Pages
111 – 116
Language
English
Abstract
The aim of the research was to study and compare morphogenetic capacity of grape explants (cultivars Regent, Marquette, Ventura, Zilga, Agat Donskoy, and Aleshenkin) during dormancy and active growth periods at the initiation stage of in vitro culture in conditions of the Republic of Belarus.
Previously, the plants had been tested for the main sap-transmittable viruses of grape: GFLV, GLRaV-1, GLRaV-2, GLRaV-3, GVA, and GFkV. The explants were buds without cover scales from hardwood cuttings in dormancy period; and buds without cover scales from softwood cuttings in period of active growth.
The sterilization scheme: main sterilizing agent 30% hydrogen peroxide; in active growth period, the exposure was 10 min, in dormancy period 15 min.
The initiation medium was modified Murashige and Skoog medium with 1.1 mg L‑1, 6-BA and 0.09 mg L‑1 NAA. During the study of explant morphophysiological capacity of grape cultivars at dormancy period, it was established that plant genotypes with high degree of reliability had determined their ability to regeneration processes in in vitro culture (p<0.001). Cultivars Agat Donskoy and Regent have maximum capacity in selected conditions (100%). Cultivars Zilga and Ventura have maximum percent of not developed explant: 69.5 and 65.0%, respectively.
In addition, callus was actively formed at the base of 41.7% of explants of cultivar Marquette. Highly reliable influence of period of initiation on regeneration processes of explants of studied cultivars Agat Donskoy, Regent and Marquette was detected (p<0.001). Activation of regeneration processes of explants on studied medium was noted only in dormancy period.
In dormancy period, the number of explants which have developed in plants was 72.2%, which is 1.9 times higher than in period of active growth.
The number of undeveloped explants was not more than 13.9%.
Previously, the plants had been tested for the main sap-transmittable viruses of grape: GFLV, GLRaV-1, GLRaV-2, GLRaV-3, GVA, and GFkV. The explants were buds without cover scales from hardwood cuttings in dormancy period; and buds without cover scales from softwood cuttings in period of active growth.
The sterilization scheme: main sterilizing agent 30% hydrogen peroxide; in active growth period, the exposure was 10 min, in dormancy period 15 min.
The initiation medium was modified Murashige and Skoog medium with 1.1 mg L‑1, 6-BA and 0.09 mg L‑1 NAA. During the study of explant morphophysiological capacity of grape cultivars at dormancy period, it was established that plant genotypes with high degree of reliability had determined their ability to regeneration processes in in vitro culture (p<0.001). Cultivars Agat Donskoy and Regent have maximum capacity in selected conditions (100%). Cultivars Zilga and Ventura have maximum percent of not developed explant: 69.5 and 65.0%, respectively.
In addition, callus was actively formed at the base of 41.7% of explants of cultivar Marquette. Highly reliable influence of period of initiation on regeneration processes of explants of studied cultivars Agat Donskoy, Regent and Marquette was detected (p<0.001). Activation of regeneration processes of explants on studied medium was noted only in dormancy period.
In dormancy period, the number of explants which have developed in plants was 72.2%, which is 1.9 times higher than in period of active growth.
The number of undeveloped explants was not more than 13.9%.
Authors
T. Krasinskaya, A. Zmushko
Keywords
grape, sterilization of explants, Vitis L., in vitro establishment
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