Articles
SSR-fingerprinting and genetic relationship study of modern peach cultivars from the germplasm collection of Nikita Botanical Gardens
Article number
1324_48
Pages
315 – 320
Language
English
Abstract
A sub collection of 40 peach (Prunus persica (L.) Batsch) cultivars from the collection of genetic resources of Nikita Botanical Gardens was evaluated by microsatellite DNA-markers for estimation of genetic diversity and relationship within the studied group of cultivars.
In total 12 SSR markers were used for genotyping.
Markers showed different levels of polymorphism: from three (for markers UDP98-410 and BPPCT028) to nine (BPPCT017) alleles per locus with mean value 5.417 alleles per locus while number of effective alleles varied from 3.970 to 1.261 with mean value 2.512. Expected and observed heterozygosity varied within the ranges 0.207-0.748 and 0.075-0.875, respectively.
According to the level of polymorphism several markers are recommended as perspective for use in further studies of genetic diversity.
For all studied cultivars unique SSR-fingerprints were obtained.
According to results of UPGMA-analysis three main groups of cultivars may be determined.
By the way few cultivars were separated into out groups.
These are: Chinese cultivar Dzyu-yus-tszyuy, which was determined as the most genetically distanced from all of the cultivars and additionally two cultivars Dostoyniy (breeding of the Nikita Botanical Gardens) and Andrey Lupan (Moldavian breeding) which both have specific fruit traits highly crispy and low melting flesh.
Distribution in the main three clusters in some cases is corresponding with genealogy of cultivars.
The obtained data showed genetic relationship among studied peach cultivars that represent the modern assortment from the collection of the Nikita Botanical Gardens.
In total 12 SSR markers were used for genotyping.
Markers showed different levels of polymorphism: from three (for markers UDP98-410 and BPPCT028) to nine (BPPCT017) alleles per locus with mean value 5.417 alleles per locus while number of effective alleles varied from 3.970 to 1.261 with mean value 2.512. Expected and observed heterozygosity varied within the ranges 0.207-0.748 and 0.075-0.875, respectively.
According to the level of polymorphism several markers are recommended as perspective for use in further studies of genetic diversity.
For all studied cultivars unique SSR-fingerprints were obtained.
According to results of UPGMA-analysis three main groups of cultivars may be determined.
By the way few cultivars were separated into out groups.
These are: Chinese cultivar Dzyu-yus-tszyuy, which was determined as the most genetically distanced from all of the cultivars and additionally two cultivars Dostoyniy (breeding of the Nikita Botanical Gardens) and Andrey Lupan (Moldavian breeding) which both have specific fruit traits highly crispy and low melting flesh.
Distribution in the main three clusters in some cases is corresponding with genealogy of cultivars.
The obtained data showed genetic relationship among studied peach cultivars that represent the modern assortment from the collection of the Nikita Botanical Gardens.
Authors
I. Suprun, A.V. Smykov, I. Stepanov, S. Tokmakov, O.S. Fedorova, Iu.A. Ivashchenko
Keywords
peach, genetic diversity, SSR-markers, DNA-fingerprints
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