Home > VIII International Scientific and Practical Conference on Biotechnology as an Instrument for Plant Biodiversity Conservation (Physiological, Biochemical, Embryological, Genetic and Legal Aspects) > Marker-free tomato plants expressed the supersweet protein thaumatin II gene

Articles

Marker-free tomato plants expressed the supersweet protein thaumatin II gene

Article number

1324_53

Pages

347 – 350

Language

English

Abstract

In our work previously we used the pMF1 vector containing the recombinase R and a CodA-nptII bifunctional selectable gene to produce tomato plants carrying the supersweet thaumatin II gene from a tropical plant under the control of fruit-specific tomato E8 or ELIP gene promoter and the tomato rubisco gene terminator.
After Agrobacterium transformation delayed strategy was followed for the selection of marker-free transgenic tomato plants.
One fully marker-free transgenic line was obtained.
With the PCR analysis we confirmed that one completely marker-free transgenic tomato plant was obtained.
The thaumatin II gene expression has been detected by RT-PCR in the primary transgenic plants as well as marker-free tomatoes.

Publication

VIII International Scientific and Practical Conference on Biotechnology as an Instrument for Plant Biodiversity Conservation (Physiological, Biochemical, Embryological, Genetic and Legal Aspects)

Authors

V. Timerbaev, S. Dolgov

Keywords

Solanum lycopersicum, fruit-specific promoter, E8, ELIP, RT-PCR

Full text

https://doi.org/10.17660/ActaHortic.2021.1324.53

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