Articles
Detection and partial molecular characterization of viruses infecting chrysanthemum
Article number
1324_49
Pages
321 – 328
Language
English
Abstract
Chrysanthemum virus B (CVB) and Tomato aspermy virus (TAV) were identified in the cultivar collection of chrysanthemum (Chrysanthemum morifolium Ramat.) maintained in the Nikita Botanical Gardens (Yalta, Russia) by reverse transcription polymerase chain reaction (RT-PCR). Total RNA was extracted from leaves of 12 cultivars displaying symptoms of suspected virus infection using RNeasy Plant Mini Kit (Qiagen). CVB was detected in local and introduced cultivars Zolotovoloska, Antonov, Simfonia, Dalystar Yellow, Diana, Chita, Demurral Lilac and hybrid 7-15. In RT-PCR with the virus-specific primers P1/P2 targeting the coat protein (CP) gene, expected products of 948 bp were amplified.
The isolates from different cultivars shared 92.0 to 98.5% identity at the nucleotide level.
Phylogenetic analysis of CP sequences available in GenBank assigned the Russian isolates in two groups with 99% bootstrap support.
Isolates from cultivars Diana, Dalystar Yellow, Antonov and hybrid 7-15 were clustered with the Indian isolates Guwahati (AJ812735) and Haryana (AJ629843). Those from Zolotovoloska, Chita, Demurral Lilac and Simfonia were grouped with the Indian isolates Bangalore (AJ585240), Chail (AJ876635) and isolate S60150 from Germany.
TAV was detected in cultivars Simfonia, Yunost and hybrid 7-15 using both virus-specific primers and primers specific to the group of bromoviruses/ cucumoviruses.
Expected PCR products of about 650 and 350 bp, respectively, were generated.
The TAV CP sequences from three cultivars were identical to one another and also the Hungarian isolate P-TAV from pepper (L15335) and the Australian isolate C-TAV from chrysanthemum (AJ277268). No CVB or TAV was detected in cultivars Sirenevye Dali, Skazka and Yuzhnaya Notch. This is the first molecular characterization of TAV and CVB infecting chrysanthemum in Russia.
The isolates from different cultivars shared 92.0 to 98.5% identity at the nucleotide level.
Phylogenetic analysis of CP sequences available in GenBank assigned the Russian isolates in two groups with 99% bootstrap support.
Isolates from cultivars Diana, Dalystar Yellow, Antonov and hybrid 7-15 were clustered with the Indian isolates Guwahati (AJ812735) and Haryana (AJ629843). Those from Zolotovoloska, Chita, Demurral Lilac and Simfonia were grouped with the Indian isolates Bangalore (AJ585240), Chail (AJ876635) and isolate S60150 from Germany.
TAV was detected in cultivars Simfonia, Yunost and hybrid 7-15 using both virus-specific primers and primers specific to the group of bromoviruses/ cucumoviruses.
Expected PCR products of about 650 and 350 bp, respectively, were generated.
The TAV CP sequences from three cultivars were identical to one another and also the Hungarian isolate P-TAV from pepper (L15335) and the Australian isolate C-TAV from chrysanthemum (AJ277268). No CVB or TAV was detected in cultivars Sirenevye Dali, Skazka and Yuzhnaya Notch. This is the first molecular characterization of TAV and CVB infecting chrysanthemum in Russia.
Authors
A.V. Zakubanskiy, I.V. Mitrofanova, N.V. Smykova, O.V. Mitrofanova, S.N. Chirkov
Keywords
Chrysanthemum morifolium, chrysanthemum virus B, Tomato aspermy virus, phylogenetic analysis
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