Articles
Some biochemical features of Olea europaea L. frost resistance
Article number
1324_61
Pages
395 – 400
Language
English
Abstract
The effect of freezing temperatures on the biochemical parameters of leaves in Olea europaea L. cultivars in the collection of Nikita Botanical Gardens was studied.
The objects of the research were: frost-resistant cultivar Nikitskaya, slightly frost-resistant cultivars Askolyana, Razzo, Correggiolo and subspecies O. europaea subsp. cuspidata. In the studies of biochemical parameters under controlled conditions, the method of cuttings cooling in a climate control chamber was used.
In the olive leaves collected in field, peroxidase activity was 1.13-1.58 a.u. g-1 s‑1. The maximum activity of this enzyme was noted in the frost-resistant cultivar Nikitskaya. After the exposure of freezing temperatures, peroxidase activity increased in most the studied samples, except the cultivar Nikitskaya (the activity was unchanged). The activity of polyphenol oxidase after freezing increased in all the studied cultivars.
The greatest increase in enzyme activity was observed in O. europaea subsp. cuspidata and Correggiolo cultivar.
Catalase activity, in the experiment treatments, changed diversely.
In the cultivars Ascolana and ‘Correggiolo’, catalase activity decreased, in the cultivars Nikitskaya, Razzo and subspecies O. europaea subsp. cuspidate it increased.
The decrease in catalase activity in the cultivars Ascolana and Correggiolo was, probably, associated with a significant increase in peroxidase activity and was due to the competition of these enzymes over the substrate.
In the olive cultivars leaves collected in field, the total content of phenolic compounds was 915-1,282 mg 100 g‑1, flavonoids 860-1,062 mg 100 g‑1, proline 15-24 μg g‑1. The effect of low-temperature stress did not cause significant changes in the content of phenolic compounds and flavonoids, but it resulted in a significant increase in proline concentration.
Thus, a precise correlation between the frost resistance of the studied O. europaea cultivars with the changes in redox enzymes activity and proline content was revealed.
The presented data suggested the participation of the above mentioned biochemical indicators in the process of European olive adaptation to the effect of freezing temperatures.
The objects of the research were: frost-resistant cultivar Nikitskaya, slightly frost-resistant cultivars Askolyana, Razzo, Correggiolo and subspecies O. europaea subsp. cuspidata. In the studies of biochemical parameters under controlled conditions, the method of cuttings cooling in a climate control chamber was used.
In the olive leaves collected in field, peroxidase activity was 1.13-1.58 a.u. g-1 s‑1. The maximum activity of this enzyme was noted in the frost-resistant cultivar Nikitskaya. After the exposure of freezing temperatures, peroxidase activity increased in most the studied samples, except the cultivar Nikitskaya (the activity was unchanged). The activity of polyphenol oxidase after freezing increased in all the studied cultivars.
The greatest increase in enzyme activity was observed in O. europaea subsp. cuspidata and Correggiolo cultivar.
Catalase activity, in the experiment treatments, changed diversely.
In the cultivars Ascolana and ‘Correggiolo’, catalase activity decreased, in the cultivars Nikitskaya, Razzo and subspecies O. europaea subsp. cuspidate it increased.
The decrease in catalase activity in the cultivars Ascolana and Correggiolo was, probably, associated with a significant increase in peroxidase activity and was due to the competition of these enzymes over the substrate.
In the olive cultivars leaves collected in field, the total content of phenolic compounds was 915-1,282 mg 100 g‑1, flavonoids 860-1,062 mg 100 g‑1, proline 15-24 μg g‑1. The effect of low-temperature stress did not cause significant changes in the content of phenolic compounds and flavonoids, but it resulted in a significant increase in proline concentration.
Thus, a precise correlation between the frost resistance of the studied O. europaea cultivars with the changes in redox enzymes activity and proline content was revealed.
The presented data suggested the participation of the above mentioned biochemical indicators in the process of European olive adaptation to the effect of freezing temperatures.
Authors
A.E. Paliy, I.N. Paliy
Keywords
Olea europaea, enzyme activity, phenolic compounds, flavonoids, proline, low temperature stress
Online Articles (68)