Articles
Low oxygen stress effect on the metabolism of pear fruit (Pyrus communis Conference)
Article number
1386_36
Pages
269 – 276
Language
English
Abstract
A thorough understanding of the metabolism of the fruit during the stress inducing low oxygen storage can provide knowledge on their adaptive survival ability.
Stable isotope labelling has been extensively used in plants for the elucidation of metabolic pathways and their regulation under biotic and abiotic stress.
To elucidate the striking adaptation behaviour of pear fruit a 13C-labelling feeding experiment was performed.
Pear tissue discs from early, commercial and late maturity stages were exposed to 21% of O2 for 24 h followed by 0.2% O2 levels for another 24 h under a constant temperature of 18°C in a liquid medium containing U-13C sodium pyruvate.
Changes in concentrations and 13C enrichment of citric acid cycle, fermentation, amino acid and γ-aminobutyric acid shunt associated metabolites were monitored to compare the metabolic effects under normoxic and hypoxic conditions and the propagation of label through the metabolic pathways.
Primary results showed successful utilization of 13C pyruvate from pear tissue accompanied by rise in organic acids such as fumarate, succinate and 2-ketoglutarate with a very small increase in lactate during hypoxia, while in contrast, sugar alcohols and sugars except glucose decreased.
Hypoxia led to a reduction of abundances of most amino acids in early and commercial stages, however, alanine was slightly increased in late maturity stages.
No γ-aminobutyric acid accumulation was seen under hypoxic treatment.
Stable isotope labelling has been extensively used in plants for the elucidation of metabolic pathways and their regulation under biotic and abiotic stress.
To elucidate the striking adaptation behaviour of pear fruit a 13C-labelling feeding experiment was performed.
Pear tissue discs from early, commercial and late maturity stages were exposed to 21% of O2 for 24 h followed by 0.2% O2 levels for another 24 h under a constant temperature of 18°C in a liquid medium containing U-13C sodium pyruvate.
Changes in concentrations and 13C enrichment of citric acid cycle, fermentation, amino acid and γ-aminobutyric acid shunt associated metabolites were monitored to compare the metabolic effects under normoxic and hypoxic conditions and the propagation of label through the metabolic pathways.
Primary results showed successful utilization of 13C pyruvate from pear tissue accompanied by rise in organic acids such as fumarate, succinate and 2-ketoglutarate with a very small increase in lactate during hypoxia, while in contrast, sugar alcohols and sugars except glucose decreased.
Hypoxia led to a reduction of abundances of most amino acids in early and commercial stages, however, alanine was slightly increased in late maturity stages.
No γ-aminobutyric acid accumulation was seen under hypoxic treatment.
Authors
K. Terzoudis, R. Kusma, M.L.A.T.M. Hertog, B.M. Nicolaï
Keywords
conference pear, maturity stages, hypoxic stress, metabolomics, 13C-label, GC-MS analysis
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