QTL mapping utilizing F_2:3 linkage mapping populations reveals regions of chromosomes 2 and 6 are significantly associated with root width in carrot

This study aimed to map quantitative trait loci (QTL) associated with carrot root width using linkage mapping.
Two bi-parental populations derived from the cross of carrot inbred lines L1408 and W133, followed by two generations of selfing, were combined resulting in a total of 123 F₃ individuals grown in a randomized complete block design in the years 2020 and 2021 in Randolph, Wisconsin, USA. Phenotyping of carrot root traits was performed using a digital imaging pipeline, and genotyping was done using genotyping-by-sequencing (GBS). A total of 3,953 high quality single nucleotide polymorphisms (SNPs) markers were retained.
A genetic map was constructed, and composite interval mapping was performed.
The results showed that three QTLs were significantly associated with carrot root width on chromosomes 2 and 6 and explain between 10 and 18% of the phenotypic variance.
The results here expand the current understanding of the genetic control of carrot root shape traits.

III International Symposium on Carrot and Other Apiaceae

A. Vega-Alfaro, S.H. Brainard, I.L. Goldman

linkage mapping, QTL mapping, Daucus carota, genotyping-by-sequencing

https://doi.org/10.17660/ActaHortic.2024.1393.24