New biomarker approach for RKN resistance in Prunus progenies

The root-knot nematodes (RKNs) Meloidogyne spp. are considered a major threat to many crops worldwide.
Three RKN resistance genes have been identified in three different Prunus species: Ma in plum, RMia in peach, and RMja in almond.
The identification of intra-gene markers for the two nematode-resistance genes Ma and RMia have led to marker-assisted selection for these two genes.
In particular, RMia confers resistance to M. incognita and M. arenaria. Currently, three specific markers are available for RMia found in the peach ‘Nemared’, and located in LG2 in the Prunus map. R genes such as RMia encode proteins of the Nucleotide type Binding and Leucine-Rich Repeat (NB-LRR). In this work, an in-silico characterization of the genomic region where R gene is located was performed by analyzing different genotypes from resistant and susceptible rootstocks.
Then, an InDel was found in resistant genotypes that will be useful to identify a candidate resistance biomarker closer to the RMia gene in Prunus progenies.

VIII International Symposium on Almonds and Pistachios

B. Bielsa, M.J. Rubio-Cabetas, J. Grimplet

biotic stress, deletion, nematodes, SAM

https://doi.org/10.17660/ActaHortic.2024.1406.49