Articles
Micropropagation of Limbarda crithmoides (L.) Dumort. and the effect of sodium chloride
Article number
1437_57
Pages
441 – 445
Language
English
Abstract
Limbarda crithmoides (L.) Dumort. is a small, halophytic shrub found naturally growing on the coasts of Greece with potential ornamental value.
The present study examined the development of an in vitro propagation protocol for the in vitro response and exploitation of the species.
Explants were obtained from pot-grown plants under protected cultivation.
After disinfection, they were cultured on two different solid nutrient media (8 g L‑1 agar): a) Woody Plant Medium (WPM) and b) Driver and Kuniyaki Walnut (DKW). Furthermore, Rugini Olive Medium (OM) was used to study the in vitro culture effect of sodium chloride (NaCl) at 0 (control), 1, 10 or 20 mg L‑1. In the initial culture the presence or absence (control, Hf) of 0.5 mg L‑1 6-benzyladenine (BA) was examined.
Subsequently, two subcultures were carried out: a) the first subculture used the same media with the initial culture, and b) the second subculture examined the in vitro effect of 0.5 mg L‑1 kinetin (KIN). During the initial culture, shooting percentage was high (85.7-97.6%); each explant contained one shoot that exceeded 0.5 cm in length, while 1.2-1.9 ‘short’ shoots (<0.5 cm) were recorded.
The mean shoot length was increased (2.6 cm) on WPM containing 0.5 mg L‑1 BA. Similar results were obtained in the first and the second subculture.
The use of OM Hf or containing 1 g L‑1 NaCl affected the elongation of the micro-shoots (2.5 and 3.8 cm, respectively). Moreover, the use of 10 g L‑1 NaCl led to the sprouting of well-formed and elongated micro-shoots with no significant difference (at P<0.05) from the micro-shoots formed on the control media (2.5 and 2.2 cm, respectively). Higher concentrations of NaCl inhibited shoot length (0.5 cm) and reduced the proliferation rate.
Spontaneous root formation was observed during all the stages registering at 100% during the second subculture.
Rooted plantlets were successfully acclimatized, and their survival percentage was 100% under protected cultivation.
The present study examined the development of an in vitro propagation protocol for the in vitro response and exploitation of the species.
Explants were obtained from pot-grown plants under protected cultivation.
After disinfection, they were cultured on two different solid nutrient media (8 g L‑1 agar): a) Woody Plant Medium (WPM) and b) Driver and Kuniyaki Walnut (DKW). Furthermore, Rugini Olive Medium (OM) was used to study the in vitro culture effect of sodium chloride (NaCl) at 0 (control), 1, 10 or 20 mg L‑1. In the initial culture the presence or absence (control, Hf) of 0.5 mg L‑1 6-benzyladenine (BA) was examined.
Subsequently, two subcultures were carried out: a) the first subculture used the same media with the initial culture, and b) the second subculture examined the in vitro effect of 0.5 mg L‑1 kinetin (KIN). During the initial culture, shooting percentage was high (85.7-97.6%); each explant contained one shoot that exceeded 0.5 cm in length, while 1.2-1.9 ‘short’ shoots (<0.5 cm) were recorded.
The mean shoot length was increased (2.6 cm) on WPM containing 0.5 mg L‑1 BA. Similar results were obtained in the first and the second subculture.
The use of OM Hf or containing 1 g L‑1 NaCl affected the elongation of the micro-shoots (2.5 and 3.8 cm, respectively). Moreover, the use of 10 g L‑1 NaCl led to the sprouting of well-formed and elongated micro-shoots with no significant difference (at P<0.05) from the micro-shoots formed on the control media (2.5 and 2.2 cm, respectively). Higher concentrations of NaCl inhibited shoot length (0.5 cm) and reduced the proliferation rate.
Spontaneous root formation was observed during all the stages registering at 100% during the second subculture.
Rooted plantlets were successfully acclimatized, and their survival percentage was 100% under protected cultivation.
Publication
Authors
A. Paraskevopoulou, K. Bertsouklis, Z. Pipida
Keywords
spontaneous root formation, ornamental, halophytes, protected cultivation, salinity
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