Articles
Identification of Xanthomonas arboricola pv. corylina strains isolated from hazelnut (Corylus avellana) in Montenegro
Article number
1449_39
Pages
293 – 298
Language
English
Abstract
Due to market demands and favorable climate, hazelnut production has increased in Montenegro recently.
However, yield is occasionally affected by different hazelnut pathogens.
Bacterial blight is one of the most damaging diseases of hazelnut worldwide.
In June 2021, leaf spot, bud and twig necrosis were observed on young hazelnut plants (Corylus avellana) cultivar ‘Hall’s Giant’, near Cetinje, central Montenegro.
From symptomatic samples, yellow, convex and mucoid bacterial colonies were isolated.
Fourteen strains were selected based on their hypersensitivity in pelargonium.
Pathogenicity of the strains was tested by spraying young leaves of potted hazelnut plants with the bacterial suspension (108 CFU mL-1 SDW). The reference strain Xanthomonas arboricola pv. corylina (Xac) NCPPB 3037 and sterile distilled water (SDW) were used as positive and negative control, respectively.
Lesions appeared on leaves of all inoculated plants within 5 to 6 weeks after inoculation, while leaves sprayed with SDW remained symptomless.
All the strains were Gram-negative, catalase positive, oxidase negative, obligate aerobic, hydrolyzed starch, gelatin and esculin, did not reduce nitrate and did not grow at 37°C and in the presence of 5% NaCl, showing the same biochemical profile as the reference Xac strain.
PCR with XarbQ-F/XarbQ-R primers produced a fragment of 402 bp in 14 strains and the reference Xac, confirming their affiliation to X. arboricola species.
Additionally, PCR analysis with primers XapY17-F/ XapY17-R, produced a single band of 943 bp.
Amplification and sequencing of the partial rpoD gene of two selected strains (GenBank Nos.
OQ271224 and OQ271225), showed that the strains share 99.47 to 99.92% rpoD sequence identity with Xac strains CP076619.1 and HG992342.1 isolated from hazelnut in France and HG992341.1 in USA. Therefore, the strains isolated from hazelnut in Montenegro were identified as Xanthomonas arboricola pv. corylina.
However, yield is occasionally affected by different hazelnut pathogens.
Bacterial blight is one of the most damaging diseases of hazelnut worldwide.
In June 2021, leaf spot, bud and twig necrosis were observed on young hazelnut plants (Corylus avellana) cultivar ‘Hall’s Giant’, near Cetinje, central Montenegro.
From symptomatic samples, yellow, convex and mucoid bacterial colonies were isolated.
Fourteen strains were selected based on their hypersensitivity in pelargonium.
Pathogenicity of the strains was tested by spraying young leaves of potted hazelnut plants with the bacterial suspension (108 CFU mL-1 SDW). The reference strain Xanthomonas arboricola pv. corylina (Xac) NCPPB 3037 and sterile distilled water (SDW) were used as positive and negative control, respectively.
Lesions appeared on leaves of all inoculated plants within 5 to 6 weeks after inoculation, while leaves sprayed with SDW remained symptomless.
All the strains were Gram-negative, catalase positive, oxidase negative, obligate aerobic, hydrolyzed starch, gelatin and esculin, did not reduce nitrate and did not grow at 37°C and in the presence of 5% NaCl, showing the same biochemical profile as the reference Xac strain.
PCR with XarbQ-F/XarbQ-R primers produced a fragment of 402 bp in 14 strains and the reference Xac, confirming their affiliation to X. arboricola species.
Additionally, PCR analysis with primers XapY17-F/ XapY17-R, produced a single band of 943 bp.
Amplification and sequencing of the partial rpoD gene of two selected strains (GenBank Nos.
OQ271224 and OQ271225), showed that the strains share 99.47 to 99.92% rpoD sequence identity with Xac strains CP076619.1 and HG992342.1 isolated from hazelnut in France and HG992341.1 in USA. Therefore, the strains isolated from hazelnut in Montenegro were identified as Xanthomonas arboricola pv. corylina.
Publication
Authors
A. Obradović, T. Popović, J. Adamović, A. Prokić, M. Ivanović
Keywords
Xanthomonas arboricola pv. corylina, bacterial blight, hazelnut, identification, rpoD
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