Articles
” VITRIFICATION ” : REVIEW OF LITERATURE
It seems that this perturbation is restricted to the in vitro culture conditions.
The radom occurence of this phenomenon makes impossible the planification of a long term industrial production.
The autors present a synthesis of morphological, physiological and biochemical characteristics of vitrified microplants.
They revieuw the different factors involved in vitrification process.
It appears clearly that vitrification results from metabolic perturbation(s) (e.g. hypolignification, water hyperhydricity…) induced by the combined action of several physical (e.g. gel solidity, temperature…) and chemical factors (e.g. amonium, cytokinines…) of the cultural environnement.
An analysis of proposed remedies to avoid vitrification reveals that none gives a satisfactory solution to this problem.
One of the most important difficulties to clarify the physiological causes of vitrification is related to the multiplicity of the factors involved in the process.
To progress in the study of vitrification it seems necessary to use simplified models providing, with a high reproductibility, vitrified plants on one hand and normal plants on the other in standardized conditions.