Articles
PROBLEMS OF SOIL ADAPTATION IN PLANTLETS OF COFFEA ARABICA L. OBTAINED VIA SOMATIC EMBRYOGENESIS
Article number
212_47
Pages
315 – 321
Language
Abstract
Clonal propagation of Coffea arabica L. can be achieved by induced somatic embryogenesis in vitro cultures of mature leaf explants.
The protocol for obtaining perfect embryos bearing a bipolarity (shoot and root poles) consisted of a primary culture on a "conditioning medium" followed by culture on an "induction medium" as described by Söndahl and Sharp (1977). Afterwards we have used, instead of a solid medium, liquid media for further growth of these embryos.
Experiments were conducted in order to see the effects of various plant growth regulators: benzylaminopurine (BAP); gibberellic acid (GA3); indole acetic acid (IAA) and naphtalene acetic acid (NAA) on the morphology of the derived plantlets.
It was found that GA3 gives a good development of the hypocotyl but reduces cotyledonary leaves; BAP at optimal concentration (1.5 mg/l) gives a drastic increase of the cotyledonary area; IAA and NAA promote root development.
In the selected medium (1.5 mg/l BAP; 1.0 mg/l GA3 and 1.0 mg/l IAA) plantlets grown under illumination at 25°C mimic normal seedlings.
Only plantlets that have a good development of the cotyledonary leaves survive when transferred directly from the liquid media into the soil.
The protocol for obtaining perfect embryos bearing a bipolarity (shoot and root poles) consisted of a primary culture on a "conditioning medium" followed by culture on an "induction medium" as described by Söndahl and Sharp (1977). Afterwards we have used, instead of a solid medium, liquid media for further growth of these embryos.
Experiments were conducted in order to see the effects of various plant growth regulators: benzylaminopurine (BAP); gibberellic acid (GA3); indole acetic acid (IAA) and naphtalene acetic acid (NAA) on the morphology of the derived plantlets.
It was found that GA3 gives a good development of the hypocotyl but reduces cotyledonary leaves; BAP at optimal concentration (1.5 mg/l) gives a drastic increase of the cotyledonary area; IAA and NAA promote root development.
In the selected medium (1.5 mg/l BAP; 1.0 mg/l GA3 and 1.0 mg/l IAA) plantlets grown under illumination at 25°C mimic normal seedlings.
Only plantlets that have a good development of the cotyledonary leaves survive when transferred directly from the liquid media into the soil.
Authors
M.L. Guimarães, M.C. Pimenta, J. Montezuma-de-Carvalho
Keywords
Online Articles (130)