Articles
PROLIFERATION AND REGENERATION OF NERINE IN LIQUID CULTURE
Article number
325_64
Pages
467 – 474
Language
Abstract
The natural propagation rate of bulb forming Amaryllidaceae including Nerine is low.
Conventional micropropagation techniques are labour intensive and therefore expensive.
Liquid cultures facilitate scaling up, automation and cost reduction of micropropagation.
Inflorescence-derived explants of N. x mansellii were cultured on 2, 4-D and BA supplemented MS medium.
Callus-like tissue interspersed with nodular regions, as well as, direct organogenesis developed at the junction between flower pedicel and the peduncle.
Subculture of nodular tissue to NAA, BA and paclobutrazol (PAC) supplemented liquid media in Erlenmeyer flasks or bubble bioreactors resulted in proliferation of compact but friable meristematic clusters.
Regeneration of plantlets from these was very sporadic.
Removal of PAC from proliferation medium induced development of proembryogenic masses.
Differentiation of somatic embryos was enhanced by 2iP and these germinated in growth regulator-free media.
From these a conversion rate of 40–50 plantlets per -3g of embryogenic tissue was obtained.
Plantlets developed rooted bulblets which were easily transferred to ex-vitro conditions.
The procedure using large scale liquid cultures for the production of embryogenic tissue is a potentially practical method for mass propagation of Nerine bulbs at a reasonable price.
Conventional micropropagation techniques are labour intensive and therefore expensive.
Liquid cultures facilitate scaling up, automation and cost reduction of micropropagation.
Inflorescence-derived explants of N. x mansellii were cultured on 2, 4-D and BA supplemented MS medium.
Callus-like tissue interspersed with nodular regions, as well as, direct organogenesis developed at the junction between flower pedicel and the peduncle.
Subculture of nodular tissue to NAA, BA and paclobutrazol (PAC) supplemented liquid media in Erlenmeyer flasks or bubble bioreactors resulted in proliferation of compact but friable meristematic clusters.
Regeneration of plantlets from these was very sporadic.
Removal of PAC from proliferation medium induced development of proembryogenic masses.
Differentiation of somatic embryos was enhanced by 2iP and these germinated in growth regulator-free media.
From these a conversion rate of 40–50 plantlets per -3g of embryogenic tissue was obtained.
Plantlets developed rooted bulblets which were easily transferred to ex-vitro conditions.
The procedure using large scale liquid cultures for the production of embryogenic tissue is a potentially practical method for mass propagation of Nerine bulbs at a reasonable price.
Publication
Authors
H. Lilien-Kipnis, M. Ziv, S. Kahany, N. Azizbekov
Keywords
somatic embryogenesis, bioreactors
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