Articles
SOMACLONAL VARIATION IN CARDAMOM (ELETTARIA CARDAMOMUM MATON) DERIVED FROM AXENIC CULTURE OF JUVENILE SHOOT PRIMORDIA
Article number
330_30
Pages
235 – 242
Language
Abstract
No systematic work has been reported to quantify the phenotypic and/or genetic variation in in vitro regenerated plants of cardamom.
In this paper the results of a preliminary trial to test for the presence of tissue culture derived phenotypic variation in a popular cultivated type of cardamom in South India viz.
MBP Vazhukka, is reported.
Four lines of plants regenerated from axenic culture of juvenile shoot primordia were grown for three years and were compared with one clonally propagated parental line for plant height, number of tillers, number of leaves, leaf variations and panicle branching character.
Considerable variation was found in plant height and panicle branching character (13.67%); but not in number of tillers and number of leaves.
Since the variation in panicle characters may affect yield, the results indicate that reliable methods for early detection of somatic variants has to be introduced in the micropropagation protocol of cardamom, especially when they are produced in mass scale for distribution among farmers.
In this paper the results of a preliminary trial to test for the presence of tissue culture derived phenotypic variation in a popular cultivated type of cardamom in South India viz.
MBP Vazhukka, is reported.
Four lines of plants regenerated from axenic culture of juvenile shoot primordia were grown for three years and were compared with one clonally propagated parental line for plant height, number of tillers, number of leaves, leaf variations and panicle branching character.
Considerable variation was found in plant height and panicle branching character (13.67%); but not in number of tillers and number of leaves.
Since the variation in panicle characters may affect yield, the results indicate that reliable methods for early detection of somatic variants has to be introduced in the micropropagation protocol of cardamom, especially when they are produced in mass scale for distribution among farmers.
Authors
B.R. Reghunath, P.M. Priyadarshan
Keywords
Cardamom, micropropagation, phenotypic variation
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