Articles
THE TISSUE CULTURE AND CHEMICAL ANALYSIS OF MELITTIS MELISSOPHYLLUM L
Article number
330_34
Pages
263 – 268
Language
Abstract
Melittis melissophyllum L. (Lamiaceae) is a perennial plant grown in Europe.
Its herb has wide applications in the folk medicine (Madaus, 1938). Its chemical composition was only partly analysed (Swiatek, Chybowski, 1982; Skaltsa et al., 1989) and we found no literature information on the in vitro culture of this plant to-date.
In the present study in vitro cultures were started from seeds.
Seedlings were obtained on MS medium supplemented with GA3 and kinetin.
The most effective micropropagation was on MS medium containing zeatin.
The shoots were rooted on MS medium with IAA or IBA. Callus tissue was obtained from almost all parts of plants (grown in vitro) generally on MS medium with 2,4-D. Thin-layer chromatography analysis of butanolic fractions of ground and in vitro cultured plants showed the presence of flavonoids and phenolic acids and/or their derivatives.
Its herb has wide applications in the folk medicine (Madaus, 1938). Its chemical composition was only partly analysed (Swiatek, Chybowski, 1982; Skaltsa et al., 1989) and we found no literature information on the in vitro culture of this plant to-date.
In the present study in vitro cultures were started from seeds.
Seedlings were obtained on MS medium supplemented with GA3 and kinetin.
The most effective micropropagation was on MS medium containing zeatin.
The shoots were rooted on MS medium with IAA or IBA. Callus tissue was obtained from almost all parts of plants (grown in vitro) generally on MS medium with 2,4-D. Thin-layer chromatography analysis of butanolic fractions of ground and in vitro cultured plants showed the presence of flavonoids and phenolic acids and/or their derivatives.
Authors
E. Skrzypczak, L. Skrzypczak
Keywords
Lamiaceae, micropropagation, flavonoids, phenolic acids, thin-layer chromatography
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