Articles
ACCUMULATION OF ESSENTIAL OIL COMPONENTS IN TISSUE CULTURES OF TAGETES MINUTA L.
Article number
330_31
Pages
243 – 248
Language
Abstract
Optimal conditions for the growth of Callus cultures from Tagetes minuta L. (Asteraceae), a plant producing insecticidal materials have been established: culturing on MS medium supplemented with 0.1 mg/l NAA, at 24±1°C, continuous white light (2500 Lux), 70% relative humidity and passageing at every third week of incubation.
As was shown by gas chramatography (GC) our calli were synthesizing thiophenes, a large group of plant insecticides.
Light (LM)- and transmission electron microscopy (TEM) of the various parts of the calli revealed that the highest accumulation (and/or synthesis?) of the secretory products (among others supposedly of thyophenes) occurred in the "middle" part of the greenish compact tissue.
These observations perhaps will be important for a planned selection of cell lines with rapid growth and elevated secreting capacity.
As was shown by gas chramatography (GC) our calli were synthesizing thiophenes, a large group of plant insecticides.
Light (LM)- and transmission electron microscopy (TEM) of the various parts of the calli revealed that the highest accumulation (and/or synthesis?) of the secretory products (among others supposedly of thyophenes) occurred in the "middle" part of the greenish compact tissue.
These observations perhaps will be important for a planned selection of cell lines with rapid growth and elevated secreting capacity.
Authors
J. Ekés-Kretovics, M. Ekés, I. Gyurján, É. Héthelyi, B. Dános
Keywords
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